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231226s2024 xx |||||o 00| ||eng c |
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|a 10.1002/adma.202306922
|2 doi
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|a eng
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|a Jiang, Ruixue
|e verfasserin
|4 aut
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|a Dual-Color Real-Time Chemosensing of a Compartmentalized Reaction Network Involving Enzyme-Induced Membrane Permeation of Peptides
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|c 2024
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|a Text
|b txt
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 26.01.2024
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|a Date Revised 26.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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|a The design of synthetic systems with interrelated reaction sequences that model incipient biological complexity is limited by physicochemical tools that allow the direct monitoring of the individual processes in real-time. To mimic a simple digestion-resorption sequence, the authors have designed compartmentalized liposomal systems that incorporate extra- and intravesicular chemosensing ensembles. The extravesicular reporter pair consists of cucurbit[7]uril and methylene blue to monitor the enzymatic cleavage of short enkephalin-related peptides by thermolysin through a switch-off fluorescence response ("digestion"). Because the substrate is membrane-impermeable, but the dipeptide product is permeable, uptake of the latter into the pre-formed liposomes occurs as a follow-up process. The intravesicular chemosensing ensemble consists of i) cucurbit[8]uril, 2-anilinonaphthalene-6-sulfonic acid, and methyl viologen or ii) cucurbit[7]uril and berberine to monitor the uptake ("resorption") of the enzymatic products through the liposomal membranes by i) a switch-on or ii) a switch-off fluorescence response. The dyes are designed to allow selective optical excitation and read-out of the extra- and intravesicular dyes, which allow for dual-color chemosensing and, therefore, kinetic discrimination of the two sequential reactions
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|a Journal Article
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|a assays
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|a cucurbiturils
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|a dyes
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|a fluorescence
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|a kinetics
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|a supramolecular chemistry
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|a Fluorescent Dyes
|2 NLM
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|a Peptides
|2 NLM
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|a Bridged-Ring Compounds
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|a Nilam, Mohamed
|e verfasserin
|4 aut
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|a Hennig, Andreas
|e verfasserin
|4 aut
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| 700 |
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|a Nau, Werner M
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Advanced materials (Deerfield Beach, Fla.)
|d 1998
|g 36(2024), 4 vom: 27. Jan., Seite e2306922
|w (DE-627)NLM098206397
|x 1521-4095
|7 nnns
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|g volume:36
|g year:2024
|g number:4
|g day:27
|g month:01
|g pages:e2306922
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|u http://dx.doi.org/10.1002/adma.202306922
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