|
|
|
|
| LEADER |
01000naa a22002652 4500 |
| 001 |
NLM361936184 |
| 003 |
DE-627 |
| 005 |
20231226090209.0 |
| 007 |
cr uuu---uuuuu |
| 008 |
231226s2023 xx |||||o 00| ||eng c |
| 024 |
7 |
|
|a 10.1016/j.plaphy.2023.108006
|2 doi
|
| 028 |
5 |
2 |
|a pubmed24n1206.xml
|
| 035 |
|
|
|a (DE-627)NLM361936184
|
| 035 |
|
|
|a (NLM)37696192
|
| 035 |
|
|
|a (PII)S0981-9428(23)00517-X
|
| 040 |
|
|
|a DE-627
|b ger
|c DE-627
|e rakwb
|
| 041 |
|
|
|a eng
|
| 100 |
1 |
|
|a Zhu, Jinling
|e verfasserin
|4 aut
|
| 245 |
1 |
0 |
|a Polyphosphate promotes oxidation resistance of ppk-expressing transgenic rice in low phosphorus culture
|
| 264 |
|
1 |
|c 2023
|
| 336 |
|
|
|a Text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a ƒaComputermedien
|b c
|2 rdamedia
|
| 338 |
|
|
|a ƒa Online-Ressource
|b cr
|2 rdacarrier
|
| 500 |
|
|
|a Date Revised 21.10.2023
|
| 500 |
|
|
|a published: Print-Electronic
|
| 500 |
|
|
|a Citation Status Publisher
|
| 520 |
|
|
|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
|
| 520 |
|
|
|a Phosphorus (P) plays a crucial role in plant growth. Insufficient availability of inorganic phosphate (Pi) can significantly impact crop yields. To address this, we previously developed transgenic rice expressing the low polyphosphate kinase gene (ppk) - known as ETRS - to enhance the efficiency of P resource utilization. Previous studies have shown that ETRS thrives and presents high yields in the low P culture. ETRS and wild-type rice (WT) were cultivated to the heading stage at 15 μM of P in the low P (LP) culture and 300 μM of P in the normal culture (CK) to identify the molecular pathways behind low P tolerance. Our findings revealed that polyphosphate (polyP) significantly enhanced the growth performance of ETRS in the LP culture. This enhanced tolerance can be attributed to polyP's capacity to mitigate oxidative damage induced by LP. This was evidenced by the reduction in levels of superoxide radicals, hydrogen peroxide, and malondialdehyde. PolyP also improved the antioxidant capacity of ETRS under LP stress by regulating enzymatic antioxidants viz., superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), as well as non-enzymatic antioxidants such as ascorbate (AsA) and glutathione (GSH). In addition, transcriptomics analysis suggested that polyP synthesis positively promoted the expressions of SOD, POD, and CAT related genes and played an active role in regulating the expression of AsA-GSH cycle system related genes in ETRS in the LP culture. These results strongly support the notion that polyP within ETRS mitigates oxidative damage through enhancement of the antioxidant system, ultimately bolstering tolerance to LP conditions
|
| 650 |
|
4 |
|a Journal Article
|
| 650 |
|
4 |
|a Antioxidant mechanisms
|
| 650 |
|
4 |
|a Phosphorus deficiency stress
|
| 650 |
|
4 |
|a Polyphosphate
|
| 650 |
|
4 |
|a RNA-Sequencing
|
| 650 |
|
4 |
|a Transgenic rice
|
| 700 |
1 |
|
|a Wei, Ruping
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Xin
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a He, Di
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Jiang, Xue
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Wang, Mengmeng
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Yicheng
|e verfasserin
|4 aut
|
| 700 |
1 |
|
|a Yang, Liuyan
|e verfasserin
|4 aut
|
| 773 |
0 |
8 |
|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 203(2023) vom: 30. Okt., Seite 108006
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
|
| 773 |
1 |
8 |
|g volume:203
|g year:2023
|g day:30
|g month:10
|g pages:108006
|
| 856 |
4 |
0 |
|u http://dx.doi.org/10.1016/j.plaphy.2023.108006
|3 Volltext
|
| 912 |
|
|
|a GBV_USEFLAG_A
|
| 912 |
|
|
|a SYSFLAG_A
|
| 912 |
|
|
|a GBV_NLM
|
| 912 |
|
|
|a GBV_ILN_350
|
| 951 |
|
|
|a AR
|
| 952 |
|
|
|d 203
|j 2023
|b 30
|c 10
|h 108006
|