Heterogeneity and mitochondrial vulnerability configurate the divergent immunoreactivity of human induced microglia-like cells

Heterogeneity and mitochondrial vulnerability configurate the divergent immunoreactivity of human induced microglia-like cells

Copyright © 2023 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 255(2023) vom: 15. Okt., Seite 109756
1. Verfasser: Yonemoto, Kousuke (VerfasserIn)
Weitere Verfasser: Fujii, Fumihiko, Taira, Ryoji, Ohgidani, Masahiro, Eguchi, Katsuhide, Okuzono, Sayaka, Ichimiya, Yuko, Sonoda, Yuri, Chong, Pin Fee, Goto, Hironori, Kanemasa, Hikaru, Motomura, Yoshitomo, Ishimura, Masataka, Koga, Yuhki, Tsujimura, Keita, Hashiguchi, Takao, Torisu, Hiroyuki, Kira, Ryutaro, Kato, Takahiro A, Sakai, Yasunari, Ohga, Shouichi
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Divergence Heterogeneity Immunoreactivity Microglia Mitochondria and CD14 Lipopolysaccharides
Beschreibung
Zusammenfassung:Copyright © 2023 Elsevier Inc. All rights reserved.
Microglia play versatile roles in progression of and protection against neuroinflammatory diseases. Little is known, however, about the mechanisms underlying the diverse reactivity of microglia to inflammatory conditions. We investigated how human induced microglia-like (iMG) cells respond to innate immune ligands. Quantitative PCR showed that poly-I:C and lipopolysaccharide (LPS) activated the expression of IL1B and TNF. Immunoreactivity of iMG did not differ between controls (n = 11) and patients with neuroinflammatory diseases (n = 24). Flow cytometry revealed that CD14high cells expressed interleukin (IL) -1β after LPS treatment. Immunoblotting showed that poly-I:C and LPS differentially activated inflammatory pathways but commonly induced mitochondrial instability and the expression of pyruvate kinase isoform M2 (PKM2). Furthermore, a potent stimulator of PKM2 (DASA-58) alleviated IL-1β production after LPS treatment. These data indicate that heterogeneous cell populations and mitochondrial stability underlie the divergent immunoreactivity of human iMG in environments
Beschreibung:Date Completed 23.10.2023
Date Revised 06.11.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2023.109756