Transcriptomic and proteomic profiling reveals distinct pathogenic features of peripheral non-classical monocytes in systemic lupus erythematosus

Transcriptomic and proteomic profiling reveals distinct pathogenic features of peripheral non-classical monocytes in systemic lupus erythematosus

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 255(2023) vom: 15. Okt., Seite 109765
1. Verfasser: Stergioti, Eirini Maria (VerfasserIn)
Weitere Verfasser: Manolakou, Theodora, Sentis, George, Samiotaki, Martina, Kapsala, Noemin, Fanouriakis, Antonis, Boumpas, Dimitrios T, Banos, Aggelos
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Cell cycle Cytokines Differentiation M1 macrophages Non-classical monocytes Systemic lupus erythematosus
Beschreibung
Zusammenfassung:Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Peripheral blood monocytes propagate inflammation in systemic lupus erythematosus (SLE). Three major populations of monocytes have been recognized namely classical (CM), intermediate (IM) and non-classical monocytes (NCM). Herein, we performed a comprehensive transcriptomic, proteomic and functional characterization of the three peripheral monocytic subsets from active SLE patients and healthy individuals. Our data demonstrate extensive molecular disruptions in circulating SLE NCM, characterized by enhanced inflammatory features such as deregulated DNA repair, cell cycle and heightened IFN signaling combined with differentiation and developmental cues. Enhanced DNA damage, elevated expression of p53, G0 arrest of cell cycle and increased autophagy stress the differentiation potential of NCM in SLE. This immunogenic profile is associated with an activated macrophage phenotype of NCM exhibiting M1 characteristics in the circulation, fueling the inflammatory response. Together, these findings identify circulating SLE NCM as a pathogenic cell type in the disease that could represent an additional therapeutic target
Beschreibung:Date Revised 28.09.2023
published: Print-Electronic
Citation Status Publisher
ISSN:1521-7035
DOI:10.1016/j.clim.2023.109765