A recombinase polymerase amplification and Pyrococcus furiosus Argonaute combined method for ultra-sensitive detection of white spot syndrome virus in shrimp

© 2023 John Wiley & Sons Ltd.

Bibliographische Detailangaben
Veröffentlicht in:Journal of fish diseases. - 1998. - 46(2023), 12 vom: 27. Dez., Seite 1357-1365
1. Verfasser: Wang, Yu (VerfasserIn)
Weitere Verfasser: Chen, Yukang, Tang, Yixin, Wang, Yue, Gao, Song, Yang, Lihong, Wang, Pei
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Journal of fish diseases
Schlagworte:Journal Article PfAgo RPA WSSV aquatic disease on-site detection Recombinases
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520 |a White spot disease (WSD) in shrimp is an acute infectious disease caused by white spot syndrome virus (WSSV). WSD has seriously threatened the security of shrimp farming, causing huge economic losses worldwide. As there is currently no effective treatment for WSD, developing early detection technologies for WSSV is of great significance for the prevention. In this study, we have established a detection method for WSSV using a combination of recombinase polymerase amplification (RPA) and Pyrococcus furiosus Argonaute (PfAgo). We have achieved a detection sensitivity of single copy per reaction, which is more sensitive than the previously reported detection methods. Additionally, we have demonstrated high specificity. The entire detection process can be completed within 75 min without the need for precise thermal cyclers, making it suitable for on-site testing. The fluorescence signal generated by the reaction can be quantified using a portable fluorescence detector or observed with the naked eye under a blue light background. This study provides an ultrasensitive on-site detection method for WSSV in shrimp aquaculture and expands the application of PfAgo in the field of aquatic disease diagnosis 
650 4 |a Journal Article 
650 4 |a PfAgo 
650 4 |a RPA 
650 4 |a WSSV 
650 4 |a aquatic disease 
650 4 |a on-site detection 
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700 1 |a Chen, Yukang  |e verfasserin  |4 aut 
700 1 |a Tang, Yixin  |e verfasserin  |4 aut 
700 1 |a Wang, Yue  |e verfasserin  |4 aut 
700 1 |a Gao, Song  |e verfasserin  |4 aut 
700 1 |a Yang, Lihong  |e verfasserin  |4 aut 
700 1 |a Wang, Pei  |e verfasserin  |4 aut 
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773 1 8 |g volume:46  |g year:2023  |g number:12  |g day:27  |g month:12  |g pages:1357-1365 
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