An insight into Pisum sativum HSF gene family-Genome-wide identification, phylogenetic, expression, and analysis of transactivation potential of pea heat shock transcription factor

Copyright © 2023 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 202(2023) vom: 01. Sept., Seite 107971
1. Verfasser: Kanwar, Meenakshi (VerfasserIn)
Weitere Verfasser: Chaudhary, Chanderkant, Anand, Kumar Ankit, Singh, Shilpi, Garg, Menus, Mishra, Sumit Kumar, Sirohi, Parul, Chauhan, Harsh
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Cis-element HSF Heat stress Pea Transactivation assay Transcription factor Heat Shock Transcription Factors
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100 1 |a Kanwar, Meenakshi  |e verfasserin  |4 aut 
245 1 3 |a An insight into Pisum sativum HSF gene family-Genome-wide identification, phylogenetic, expression, and analysis of transactivation potential of pea heat shock transcription factor 
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520 |a Field pea (Pisum sativum L, 2n = 14) is a popular temperate legume with high economic value. Heat shock factors (HSFs) are the core element in the regulatory mechanism of heat stress responses. HSFs in pea (P. sativum) have not been characterized and their role remains unclear in different abiotic stresses. To address this knowledge gap, the current study aimed to characterize the HSF gene family in pea. We identified 38 PsHsf members in P. sativum, which are distributed on the seven chromosomes, and based on phylogenetic analysis, we classified them into three representative classes i.e. A, B, and C. Conserved motif and gene structure analysis confirmed a high degree of similarity among the members of the same class. Additionally, identified cis-acting regulatory elements (CAREs) related to abiotic responses, development, growth, and hormone signaling provides crucial insights into the regulatory mechanisms of PsHsfs. Our research revealed instances of gene duplication in PsHsf gene family, suggesting that this mechanism could be driving the expansion of the PsHsf gene family. Moreover, Expression analysis of PsHsfs exhibited upregulation under heat stress (HS), salt stress (SS), and drought stress (DS) showing their phenomenal role in stress conditions. PsHsfs protein interaction network suggested their involvement in stress-responsive mechanisms. Further transactivation potential was checked for spliced variant of PsHsfA2a (PsHsfA2aI, PsHsfA2aII, and PsHsfA2aIII), PsHsfA3, PsHsfA6b, PsHsfA9, PsHsfB1a, and PsHsfB2a. Overall, these findings provide valuable insight into the evolutionary relationship of PsHsf gene family and their role in abiotic stress responses 
650 4 |a Journal Article 
650 4 |a Cis-element 
650 4 |a HSF 
650 4 |a Heat stress 
650 4 |a Pea 
650 4 |a Transactivation assay 
650 4 |a Transcription factor 
650 7 |a Heat Shock Transcription Factors  |2 NLM 
700 1 |a Chaudhary, Chanderkant  |e verfasserin  |4 aut 
700 1 |a Anand, Kumar Ankit  |e verfasserin  |4 aut 
700 1 |a Singh, Shilpi  |e verfasserin  |4 aut 
700 1 |a Garg, Menus  |e verfasserin  |4 aut 
700 1 |a Mishra, Sumit Kumar  |e verfasserin  |4 aut 
700 1 |a Sirohi, Parul  |e verfasserin  |4 aut 
700 1 |a Chauhan, Harsh  |e verfasserin  |4 aut 
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773 1 8 |g volume:202  |g year:2023  |g day:01  |g month:09  |g pages:107971 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2023.107971  |3 Volltext 
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