Printed Divisional Optical Biochip for Multiplex Visualizable Exosome Analysis at Point-of-Care

Bibliographische Detailangaben
Veröffentlicht in:	Advanced materials (Deerfield Beach, Fla.). - 1998. - 36(2024), 4 vom: 05. Jan., Seite e2304935
1. Verfasser:	Yang, Xu (VerfasserIn)
Weitere Verfasser:	Zhang, Zeying, Wu, Yuanbin, Wang, Huadong, Yun, Yang, Sun, Yali, Xie, Hongfei, Bogdanov, Bogdan, Senyushkin, Pavel, Chi, Jimei, Lian, Zewei, Wu, Dongdong, Su, Meng, Song, Yanlin
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2024
Zugriff auf das übergeordnete Werk:	Advanced materials (Deerfield Beach, Fla.)
Schlagworte:	Journal Article droplet manipulation exosomes multiplex detection self-assembly Antibodies

Beschreibung
Zusammenfassung:	© 2023 Wiley-VCH GmbH. Rapid detection of various exosomes is of great significance in early diagnosis and postoperative monitoring of cancers. Here, a divisional optical biochip is reported for multiplex exosome analysis via combining the self-assembly of nanochains and precise surface patterning. Arising from resonance-induced near-field enhancement, the nanochains show distinct color changes after capturing target exosomes for direct visual detection. Then, a series of divisional nanochain-based biochips conjugated with several specific antibodies are fabricated through designed hydrophilic and hydrophobic patterns. Because of the significant wettability difference, one sample droplet is precisely self-splitting into several microdroplets enabling simultaneous identification of multiple target exosomes in 30 min with a sensitivity of 6 × 107 particles mL-1 , which is about two orders lower than enzyme-linked immunosorbent assay. Apart from the trace amount detection, excellent semiquantitative capability is demonstrated to distinguish clinical exosomes from glioblastoma patients and healthy people. This method is simple, versatile, and highly efficient that can be extended as a diagnostic tool for many diseases, promoting the development of liquid biopsy
Beschreibung:	Date Completed 26.01.2024 Date Revised 26.01.2024 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-4095
DOI:	10.1002/adma.202304935