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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c01301
|2 doi
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|a pubmed25n1201.xml
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|a (NLM)37552772
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
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|a Sharma, Anurag
|e verfasserin
|4 aut
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| 245 |
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|a Kinetics of Ganglioside-Rich Supported Lipid Bilayer Formation with Tracer Vesicle Fluorescence Imaging
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.08.2023
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|a Date Revised 24.08.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Gangliosides, forming a class of lipids complemented by sugar chains, influence the lateral distribution of membrane proteins or membrane-binding proteins, act as receptors for viruses and bacterial toxins, and mediate several types of cellular signaling. Gangliosides incorporated into supported lipid bilayers (SLBs) have been widely applied as a model system to examine these biological processes. In this work, we explored how ganglioside composition affects the kinetics of SLB formation using the vesicle rupturing method on a solid surface. We imaged the attachment of vesicles and the subsequent SLB formation using the time-lapse total internal reflection fluorescence microscopy technique. In the early phase, the ganglioside type and concentration influence the adsorption kinetics of vesicles and their residence/lifetime on the surface before rupturing. Our data confirm that a simultaneous rupturing of neighboring surface-adsorbed vesicles forms microscopic lipid patches on the surface and it is triggered by a critical coverage of the vesicles independent of their composition. In the SLB growth phase, lipid patches merge, forming a continuous SLB. The propagation of patch edges catalyzes the process and depends on the ganglioside type. Our pH-dependent experiments confirm that the polar/charged head groups of the gangliosides have a critical role in these steps and phases of SLB formation kinetics
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lipid Bilayers
|2 NLM
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|a Gangliosides
|2 NLM
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|a Bacterial Toxins
|2 NLM
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1 |
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|a Negi, Geetanjali
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chaudhary, Monika
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Parveen, Nagma
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1985
|g 39(2023), 33 vom: 22. Aug., Seite 11694-11707
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnas
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| 773 |
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|g volume:39
|g year:2023
|g number:33
|g day:22
|g month:08
|g pages:11694-11707
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|u http://dx.doi.org/10.1021/acs.langmuir.3c01301
|3 Volltext
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