WEAK SEED DORMANCY 1, an aminotransferase protein, regulates seed dormancy in rice through the GA and ABA pathways

WEAK SEED DORMANCY 1, an aminotransferase protein, regulates seed dormancy in rice through the GA and ABA pathways

Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 202(2023) vom: 01. Sept., Seite 107923
1. Verfasser: Huang, Yunshuai (VerfasserIn)
Weitere Verfasser: Song, Jiawei, Hao, Qixian, Mou, Changling, Wu, Hongming, Zhang, Fulin, Zhu, Ziyan, Wang, Ping, Ma, Tengfei, Fu, Kai, Chen, Yaping, Nguyen, Thanhliem, Liu, Shijia, Jiang, Ling, Wan, Jianmin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Oryza sativa Pre-harvest sprouting Seed germination WSD1 Gibberellins Abscisic Acid 72S9A8J5GW Transaminases EC 2.6.1.-
Beschreibung
Zusammenfassung:Copyright © 2023 The Authors. Published by Elsevier Masson SAS.. All rights reserved.
Seed dormancy is a critical trait that enhances plant survival by preventing seed germination at the wrong time or under unsuitable conditions. Lack of seed dormancy in rice can lead to pre-harvest sprouting on mother plants leading to reduced yield and seed quality. Although some genes have been identified, knowledge of regulation of seed dormancy is limited. Here, we characterized a weak seed dormancy mutant named weak seed dormancy 1 (wsd1) that showed a higher seed germination percentage than the wild-type following the harvest ripeness. We cloned the WSD1 encoding an aminotransferase protein using a MutMap approach. WSD1 was stably expressed after imbibition and its protein was localized in the endoplasm reticulum. A widely targeted metabolomics assay and amino acid analysis showed that WSD1 had a role in regulating homeostasis of amino acids. PAC treatment and RNA-seq analysis showed that WSD1 regulates seed dormancy by involvement in the GA biosynthesis pathway. GA1 content and expression of GA biosynthesis-related genes were increased in the wsd1 mutant compared with the wild-type. The wsd1 mutant had reduced sensitivity to ABA. Our overall results indicated that WSD1 regulates seed dormancy by balancing the ABA and GA pathways
Beschreibung:Date Completed 12.09.2023
Date Revised 12.09.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2023.107923