Interaction of the antiphospholipid syndrome autoantigen beta-2 glycoprotein I with DNA and neutrophil extracellular traps

Copyright © 2023 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 255(2023) vom: 01. Okt., Seite 109714
1. Verfasser: Kmeťová, Katarína (VerfasserIn)
Weitere Verfasser: Lonina, Elena, Yalavarthi, Srilakshmi, Levine, Jerrold S, Hoy, Claire K, Sarosh, Cyrus, Gockman, Kelsey, Morris, Alexandra E, Tambralli, Ajay, Madison, Jacqueline A, Zuo, Yu, Subang, Rebecca, Rauch, Joyce, Knight, Jason S
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Antiphospholipid antibodies Antiphospholipid syndrome DNA Lupus Neutrophil extracellular traps beta-2 glycoprotein I beta 2-Glycoprotein I mehr... Autoantigens 9007-49-2
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520 |a Beta-2 glycoprotein I (β2GPI) is a phospholipid-binding plasma protein and prominent autoantigen in antiphospholipid syndrome (APS). Here, we tested the hypothesis that β2GPI might bind to not only phospholipids, but also cell-free DNA and neutrophil extracellular traps (NETs). We report that β2GPI interacts with cell-free DNA from different species, as well as NETs, in a dose-dependent manner, retarding their migration in an agarose-gel electrophoretic mobility shift assay. We confirm the direct binding interaction of β2GPI with DNA and NETs by ELISA. We also demonstrate that β2GPI colocalizes with NET strands by immunofluorescence microscopy. Finally, we provide evidence that β2GPI-DNA complexes can be detected in the plasma of APS patients, where they positively correlate with an established biomarker of NET remnants. Taken together, our findings indicate that β2GPI interacts with DNA and NETs, and suggest that this interaction may play a role as a perpetuator and/or instigator of autoimmunity in APS 
650 4 |a Journal Article 
650 4 |a Research Support, N.I.H., Extramural 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Antiphospholipid antibodies 
650 4 |a Antiphospholipid syndrome 
650 4 |a DNA 
650 4 |a Lupus 
650 4 |a Neutrophil extracellular traps 
650 4 |a beta-2 glycoprotein I 
650 7 |a beta 2-Glycoprotein I  |2 NLM 
650 7 |a Autoantigens  |2 NLM 
650 7 |a DNA  |2 NLM 
650 7 |a 9007-49-2  |2 NLM 
700 1 |a Lonina, Elena  |e verfasserin  |4 aut 
700 1 |a Yalavarthi, Srilakshmi  |e verfasserin  |4 aut 
700 1 |a Levine, Jerrold S  |e verfasserin  |4 aut 
700 1 |a Hoy, Claire K  |e verfasserin  |4 aut 
700 1 |a Sarosh, Cyrus  |e verfasserin  |4 aut 
700 1 |a Gockman, Kelsey  |e verfasserin  |4 aut 
700 1 |a Morris, Alexandra E  |e verfasserin  |4 aut 
700 1 |a Tambralli, Ajay  |e verfasserin  |4 aut 
700 1 |a Madison, Jacqueline A  |e verfasserin  |4 aut 
700 1 |a Zuo, Yu  |e verfasserin  |4 aut 
700 1 |a Subang, Rebecca  |e verfasserin  |4 aut 
700 1 |a Rauch, Joyce  |e verfasserin  |4 aut 
700 1 |a Knight, Jason S  |e verfasserin  |4 aut 
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