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231226s2023 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2023-0015
|2 doi
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|e rakwb
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|a eng
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| 100 |
1 |
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|a Liu, Nathan
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A novel use of a needle-free injection system for improved nucleic acid delivery and expression in vivo
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1 |
|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 18.08.2023
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|a Date Revised 24.08.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Transfection, a nonviral method of nucleic acid delivery, often exhibits poor efficiency in vivo. The needle-based in vivo delivery of transfection reagents can be invasive. Here, we report a noninvasive protocol for in vivo gene delivery via the needle-free MED-JET H4 MULTIJET (MJH4M) device using both "home-made" glucose-based and commercial transfection reagents. The objective of this study was to compare the relative transfection efficiencies of the needle-free system to that of the needle-based delivery method. We observed a 15-fold increase in transfection efficiency using the needle-free MJH4M device when compared to the needle-based delivery method. The highest transfection efficiency was achieved using a 5% glucose solution as the delivery vehicle
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a injection
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|a luciferase
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4 |
|a needle-free
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| 650 |
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|a nucleic acid delivery
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4 |
|a transfection
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| 650 |
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7 |
|a Nucleic Acids
|2 NLM
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| 700 |
1 |
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|a Abd-Ul-Salam, Hani
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Joannette-Lafrance, Noémie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Jingjing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Menassa, Karim
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Murshed, Monzur
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t BioTechniques
|d 1991
|g 75(2023), 2 vom: 06. Aug., Seite 65-70
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
8 |
|g volume:75
|g year:2023
|g number:2
|g day:06
|g month:08
|g pages:65-70
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| 856 |
4 |
0 |
|u http://dx.doi.org/10.2144/btn-2023-0015
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