Crosstalk between TRPV1 and immune regulation in Fuchs endothelial corneal dystrophy

Crosstalk between TRPV1 and immune regulation in Fuchs endothelial corneal dystrophy

Copyright © 2023. Published by Elsevier Inc.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 254(2023) vom: 15. Sept., Seite 109701
1. Verfasser: Cai, Yuchen (VerfasserIn)
Weitere Verfasser: Chen, Jin, Sun, Hao, Zhou, Tianyi, Cai, Xueyao, Fu, Yao
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Fuchs endothelial corneal dystrophy Gene therapy Immune landscape Regulatory T cell Transient receptor potential vanilloid 1 Hydrogen Peroxide BBX060AN9V TRPV1 protein, human TRPV Cation Channels
Beschreibung
Zusammenfassung:Copyright © 2023. Published by Elsevier Inc.
Fuchs endothelial corneal dystrophy (FECD) is the leading indication for corneal transplantation worldwide. Our aim was to investigate the role of transient receptor potential vanilloid subtype 1 (TRPV1) and the associated immune regulation contributing to this pathological condition. Significant upregulation of TRPV1 was detected in the H2O2-induced in vitro FECD model. Based on gene expression microarray dataset GSE142538 and in vitro results, a comprehensive immune landscape was studied and a negative correlation was found between TRPV1 with different immune cells, especially regulatory T cells (Tregs). Functional analyses of the 313 TRPV1-related differentially expressed genes (DEGs) revealed the involvement of TRP-regulated calcium transport, as well as inflammatory and immune pathways. Four TRPV1-related core genes (MAPK14, GNB1, GNAQ, and ARRB2) were screened, validated by microarray dataset GSE112039 and the combined validation dataset E-GEAD-399 & 564, and verified by in vitro experiments. Our study suggested a potential crosstalk between TRPV1 and immune regulation contributing to FECD pathogenesis. The identified pivotal biomarkers and immune-related pathways provide a novel framework for future mechanistic and therapeutic studies of FECD
Beschreibung:Date Completed 14.08.2023
Date Revised 22.08.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2023.109701