A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors

© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 35(2023), 42 vom: 14. Okt., Seite e2304102
1. Verfasser: Genco, Enrico (VerfasserIn)
Weitere Verfasser: Modena, Francesco, Sarcina, Lucia, Björkström, Kim, Brunetti, Celestino, Caironi, Mario, Caputo, Mariapia, Demartis, Virginia Maria, Di Franco, Cinzia, Frusconi, Giulia, Haeberle, Lena, Larizza, Piero, Mancini, Maria Teresa, Österbacka, Ronald, Reeves, William, Scamarcio, Gaetano, Scandurra, Cecilia, Wheeler, May, Cantatore, Eugenio, Esposito, Irene, Macchia, Eleonora, Torricelli, Fabrizio, Viola, Fabrizio Antonio, Torsi, Luisa
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article bioelectronics biomarkers early detection pancreatic cancer precursors printed-electronics single molecules single-molecule-with-a-large-transistor Proto-Oncogene Proteins p21(ras) EC 3.6.5.2
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100 1 |a Genco, Enrico  |e verfasserin  |4 aut 
245 1 2 |a A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors 
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500 |a Date Completed 23.10.2023 
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520 |a © 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH. 
520 |a A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut , MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma 
650 4 |a Journal Article 
650 4 |a bioelectronics 
650 4 |a biomarkers 
650 4 |a early detection 
650 4 |a pancreatic cancer precursors 
650 4 |a printed-electronics 
650 4 |a single molecules 
650 4 |a single-molecule-with-a-large-transistor 
650 7 |a Proto-Oncogene Proteins p21(ras)  |2 NLM 
650 7 |a EC 3.6.5.2  |2 NLM 
700 1 |a Modena, Francesco  |e verfasserin  |4 aut 
700 1 |a Sarcina, Lucia  |e verfasserin  |4 aut 
700 1 |a Björkström, Kim  |e verfasserin  |4 aut 
700 1 |a Brunetti, Celestino  |e verfasserin  |4 aut 
700 1 |a Caironi, Mario  |e verfasserin  |4 aut 
700 1 |a Caputo, Mariapia  |e verfasserin  |4 aut 
700 1 |a Demartis, Virginia Maria  |e verfasserin  |4 aut 
700 1 |a Di Franco, Cinzia  |e verfasserin  |4 aut 
700 1 |a Frusconi, Giulia  |e verfasserin  |4 aut 
700 1 |a Haeberle, Lena  |e verfasserin  |4 aut 
700 1 |a Larizza, Piero  |e verfasserin  |4 aut 
700 1 |a Mancini, Maria Teresa  |e verfasserin  |4 aut 
700 1 |a Österbacka, Ronald  |e verfasserin  |4 aut 
700 1 |a Reeves, William  |e verfasserin  |4 aut 
700 1 |a Scamarcio, Gaetano  |e verfasserin  |4 aut 
700 1 |a Scandurra, Cecilia  |e verfasserin  |4 aut 
700 1 |a Wheeler, May  |e verfasserin  |4 aut 
700 1 |a Cantatore, Eugenio  |e verfasserin  |4 aut 
700 1 |a Esposito, Irene  |e verfasserin  |4 aut 
700 1 |a Macchia, Eleonora  |e verfasserin  |4 aut 
700 1 |a Torricelli, Fabrizio  |e verfasserin  |4 aut 
700 1 |a Viola, Fabrizio Antonio  |e verfasserin  |4 aut 
700 1 |a Torsi, Luisa  |e verfasserin  |4 aut 
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773 1 8 |g volume:35  |g year:2023  |g number:42  |g day:14  |g month:10  |g pages:e2304102 
856 4 0 |u http://dx.doi.org/10.1002/adma.202304102  |3 Volltext 
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