SARS-COV-2 specific t-cells in patients with thyroid disorders related to COVID-19 are enriched in the thyroid and acquire a tissue-resident memory phenotype

Bibliographische Detailangaben
Veröffentlicht in:	Clinical immunology (Orlando, Fla.). - 1999. - 254(2023) vom: 01. Sept., Seite 109684
1. Verfasser:	Silvestri, Ylenia (VerfasserIn)
Weitere Verfasser:	Clemente, Francesca, Moschetti, Giorgia, Maioli, Sara, Carelli, Elena, Espadas de Arias, Alejandro, Torelli, Rosanna, Longhi, Elena, De Feo, Tullia, Crosti, MariaCristina, Sarnicola, Maria Lucia, Salvi, Mario, Mantovani, Giovanna, Arosio, Maura, Bombaci, Mauro, Pesce, Elisa, Grifantini, Renata, Abrignani, Sergio, Geginat, Jens, Muller, Ilaria
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2023
Zugriff auf das übergeordnete Werk:	Clinical immunology (Orlando, Fla.)
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Anti-viral T-cells SARS-COV2 Thyroid Tissue resident memory cells RNA, Viral Antibodies

Beschreibung
Zusammenfassung:	Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved. BACKGROUND: SARS-CoV-2 infections have been associated with the onset of thyroid disorders like classic subacute thyroiditis (SAT) or atypical SAT upon severe COVID disease (COV-A-SAT). Little is known about thyroid anti-viral immune responses OBJECTIVES: To define the role of T-cells in COV-A-SAT METHODS: T-cells from COV-A-SAT patients were analyzed by multi-dimensional flow cytometry, UMAP and DiffusionMap dimensionality reduction and FlowSOM clustering. T-cells from COVID-naïve healthy donors, patients with autoimmune thyroiditis (ATD) and with SAT following COVID vaccination were analyzed as controls. T-cells were analyzed four and eight months post-infection in peripheral blood and in thyroid specimen obtained by ultrasound-guided fine needle aspiration. SARS-COV2-specific T-cells were identified by cytokine production induced by SARS-COV2-derived peptides and with COVID peptide-loaded HLA multimers after HLA haplotyping RESULTS: COV-A-SAT was associated with HLA-DRB113 and HLA-B57. COV-A-SAT patients contained activated Th1- and cytotoxic CD4+ and CD8+ effector cells four months post-infection, which acquired a quiescent memory phenotype after eight months. Anti-SARS-CoV-2-specific T-cell responses were readily detectable in peripheral blood four months post-infection, but were reduced after eight months. CD4+ and CD8+ tissue-resident memory cells (TRM) were present in the thyroid, and circulating CXCR3+T-cells identified as their putative precursors. SARS-CoV-2-specific T-cells were enriched in the thyroid, and acquired a TRM phenotype eight months post-infection CONCLUSIONS: The association of COV-A-SAT with specific HLA haplotypes suggests a genetic predisposition and a key role for T-cells. COV-A-SAT is characterized by a prolonged systemic anti-viral effector T-cell response and the late generation of COVID-specific TRM in the thyroid target tissue
Beschreibung:	Date Completed 14.08.2023 Date Revised 22.08.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1521-7035
DOI:	10.1016/j.clim.2023.109684