Agreement between hematocrit values determined by the Cobas b121 blood gas analyzer and the microhematocrit method in dogs, cats, and horses

© 2023 American Society for Veterinary Clinical Pathology.

Bibliographische Detailangaben
Veröffentlicht in:Veterinary clinical pathology. - 1975. - 52(2023), 3 vom: 12. Sept., Seite 412-416
1. Verfasser: Dallarosa, Pâmela (VerfasserIn)
Weitere Verfasser: Monteiro, Eduardo R, Borenstein, Ana Paula S, Valle, Stella F
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Veterinary clinical pathology
Schlagworte:Journal Article blood conductivity canine equine feline packed cell volume
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100 1 |a Dallarosa, Pâmela  |e verfasserin  |4 aut 
245 1 0 |a Agreement between hematocrit values determined by the Cobas b121 blood gas analyzer and the microhematocrit method in dogs, cats, and horses 
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500 |a Date Completed 12.09.2023 
500 |a Date Revised 12.09.2023 
500 |a published: Print-Electronic 
500 |a Citation Status MEDLINE 
520 |a © 2023 American Society for Veterinary Clinical Pathology. 
520 |a BACKGROUND: Packed cell volume (PCV) is important for assessing a patient's health status. Some blood gas analyzers measure hematocrit, and the agreement with PCV varies among different analyzers 
520 |a OBJECTIVES: We aimed to determine the agreement between PCV measured by microcentrifugation and hematocrit measured by the Cobas b121 blood gas analyzer in dogs, cats, and horses 
520 |a METHODS: Whole blood samples for PCV and blood gas analysis were collected in lithium-heparin syringes and analyzed within 10 min of collection. Agreement and association between the PCV and Cobas b121 generated hematocrit were assessed by the Bland-Altman method, Pearson's correlation, Deming regression analysis, and paired t tests. A total allowable error of 10% was used for the analysis 
520 |a RESULTS: This study included 45 dogs, 45 cats, and 33 horses. The respective mean ± SD (minimum-maximum) of PCVs and hematocrits were: dogs, 34.9 ± 9.9% (9.0-55.0) and 32.5 ± 8.8% (10.4-50.6); cats, 29.0 ± 9.6% (11.0-51.0) and 26.9 ± 9.3% (10.2-50.9); horses, 34.2 ± 6.5% (24.0-47.0) and 34.1 ± 6.0% (22.5-46.1). There were no significant differences between the methods. The bias ± SD was: dogs, -2.4 ± 2.6%; cats, -2.2 ± 2.3%; horses, -0.1 ± 2.4%. Pearson's correlation coefficients were > 0.90 for all species (P < 0.0001). In 60%, 49%, and 85% of the samples for dogs, cats, and horses, respectively, the percentage differences between the methods were within 10% 
520 |a CONCLUSIONS: The Cobas b121 blood gas analyzer provided accurate estimates of PCVs in horses. However, in dogs and cats, there was a large frequency of unacceptable differences between the methods 
650 4 |a Journal Article 
650 4 |a blood conductivity 
650 4 |a canine 
650 4 |a equine 
650 4 |a feline 
650 4 |a packed cell volume 
700 1 |a Monteiro, Eduardo R  |e verfasserin  |4 aut 
700 1 |a Borenstein, Ana Paula S  |e verfasserin  |4 aut 
700 1 |a Valle, Stella F  |e verfasserin  |4 aut 
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773 1 8 |g volume:52  |g year:2023  |g number:3  |g day:12  |g month:09  |g pages:412-416 
856 4 0 |u http://dx.doi.org/10.1111/vcp.13245  |3 Volltext 
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