Rhizophagus irregularis, the model fungus in arbuscular mycorrhiza research, forms dimorphic spores
© 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist published by John Wiley & Sons Ltd on behalf of New Phytologist Foundation. Reproduced with the permission of the Minister of Agriculture and Agri‐Food Canada.
| Veröffentlicht in: | The New phytologist. - 1979. - 242(2024), 4 vom: 10. Apr., Seite 1771-1784 |
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| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2024
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't Rhizophagus irregularis SSU‐ITS‐LSU rDNA region arbuscular mycorrhizal fungi glomalin gene morphology spore dimorphism spore wall layers |
| Zusammenfassung: | © 2023 His Majesty the King in Right of Canada and The Authors. New Phytologist published by John Wiley & Sons Ltd on behalf of New Phytologist Foundation. Reproduced with the permission of the Minister of Agriculture and Agri‐Food Canada. Rhizophagus irregularis is the model species for arbuscular mycorrhizal fungi (AMF) research and the most widely propagated species for commercial plant biostimulants. Using asymbiotic and symbiotic cultivation systems initiated from single spores, advanced microscopy, Sanger sequencing of the glomalin gene, and PacBio sequencing of the partial 45S rRNA gene, we show that four strains of R. irregularis produce spores of two distinct morphotypes, one corresponding to the morphotype described in the R. irregularis protologue and the other having the phenotype of R. fasciculatus. The two spore morphs are easily distinguished by spore colour, thickness of the subtending hypha, thickness of the second wall layer, lamination of the innermost layer, and the dextrinoid reaction of the two outer spore wall layers to Melzer's reagent. The glomalin gene of the two spore morphs is identical and that of the PacBio sequences of the partial SSU-ITS-LSU region (2780 bp) obtained from single spores of the R. cf fasciculatus morphotype has a median pairwise similarity of 99.8% (SD = 0.005%) to the rDNA ribotypes of R. irregularis DAOM 197198. Based on these results, we conclude that the model AMF species R. irregularis is dimorphic, which has caused taxonomic confusion in culture collections and possibly in AMF research |
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| Beschreibung: | Date Completed 25.04.2024 Date Revised 25.04.2024 published: Print-Electronic RefSeq: OQ628336, OQ628347 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19121 |