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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13825
|2 doi
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|a pubmed24n1196.xml
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|a (NLM)37409374
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Zheng, Yihua
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Development of a lateral flow immuno-chromatic strip assay for the detection of cyprinid herpesvirus 3 (CyHV-3)
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 14.09.2023
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|a Date Revised 14.09.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 John Wiley & Sons Ltd.
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|a Cyprinid herpesvirus 3 (CyHV-3) is the main pathogen of koi herpesvirus disease (KHVD), which has caused serious damage to the ornamental and food-producing carp industry. Effective and rapid on-site detection methods are needed for early diagnosis of CyHV-3. A lateral flow immuno-chromatographic assay (LFIA) using two specific anti-CyHV-3 monoclonal antibodies has been developed and validated for on-site detection of CyHV-3. MAb 3C9 was used to bio-conjugate CyHV-3 antigen with colloidal gold, and MAb 2A8 was used to capture antigen bound colloidal gold on the test line. The control line was lined with goat anti-mouse IgG to capture unbound colloidal gold to validate performance. The test results can be viewed within 10 min after putting the strip into CyHV-3 virus infection fluid. The lowest limit of detection for the LFIA test was found to be 1.5 × 104 copies/μL and it showed no cross-reactivity with other fish viral pathogens. The specificity of the strip was 100% when spleen and kidney tissues of CyHV-3-infected and healthy koi were validated at the field level. The LFIA strip will be an effective device for the early detection of CyHV-3 in the future
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|a Journal Article
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|a cyprinid herpesvirus 3
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|a lateral flow assay
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| 650 |
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4 |
|a monoclonal antibody
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| 700 |
1 |
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|a Zhou, Yi
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Lupin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Li, Jiaxun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Lu, Liqun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Yousheng
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 46(2023), 10 vom: 04. Okt., Seite 1065-1071
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
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|g volume:46
|g year:2023
|g number:10
|g day:04
|g month:10
|g pages:1065-1071
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| 856 |
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|u http://dx.doi.org/10.1111/jfd.13825
|3 Volltext
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