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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1093/jxb/erad255
|2 doi
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|a pubmed25n1196.xml
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|a (DE-627)NLM359038123
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|a (NLM)37402629
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Choi, Soyeon
|e verfasserin
|4 aut
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| 245 |
1 |
2 |
|a A single amino acid change led to structural and functional differentiation of PvHd1 to control flowering in switchgrass
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| 264 |
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|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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| 337 |
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 02.10.2023
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|a Date Revised 04.10.2023
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|a published: Print
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|a Citation Status MEDLINE
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| 520 |
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
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|a Switchgrass, a forage and bioenergy crop, occurs as two main ecotypes with different but overlapping ranges of adaptation. The two ecotypes differ in a range of characteristics, including flowering time. Flowering time determines the duration of vegetative development and therefore biomass accumulation, a key trait in bioenergy crops. No causal variants for flowering time differences between switchgrass ecotypes have, as yet, been identified. In this study, we mapped a robust flowering time quantitative trait locus (QTL) on chromosome 4K in a biparental F2 population and characterized the flowering-associated transcription factor gene PvHd1, an ortholog of CONSTANS in Arabidopsis and Heading date 1 in rice, as the underlying causal gene. Protein modeling predicted that a serine to glycine substitution at position 35 (p.S35G) in B-Box domain 1 greatly altered the global structure of the PvHd1 protein. The predicted variation in protein compactness was supported in vitro by a 4 °C shift in denaturation temperature. Overexpressing the PvHd1-p.35S allele in a late-flowering CONSTANS-null Arabidopsis mutant rescued earlier flowering, whereas PvHd1-p.35G had a reduced ability to promote flowering, demonstrating that the structural variation led to functional divergence. Our findings provide us with a tool to manipulate the timing of floral transition in switchgrass cultivars and, potentially, expand their cultivation range
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4 |
|a Journal Article
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| 650 |
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4 |
|a Research Support, U.S. Gov't, Non-P.H.S.
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| 650 |
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4 |
|a Research Support, Non-U.S. Gov't
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| 650 |
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4 |
|a Panicum virgatum
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| 650 |
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4 |
|a Bioenergy
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| 650 |
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4 |
|a CONSTANS (CO)
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| 650 |
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4 |
|a Heading date 1 (Hd1)
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| 650 |
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4 |
|a flowering time
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| 650 |
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4 |
|a latitudinal adaptation
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| 650 |
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4 |
|a protein structure and function
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| 650 |
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4 |
|a quantitative trait locus (QTL)
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| 650 |
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4 |
|a single nucleotide polymorphism (SNP)
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| 650 |
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4 |
|a switchgrass
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| 650 |
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7 |
|a Amino Acids
|2 NLM
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| 700 |
1 |
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|a Prabhakar, Pradeep K
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chowdhury, Ratul
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pendergast, Thomas H
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Urbanowicz, Breeanna R
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Maranas, Costas
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Devos, Katrien M
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 74(2023), 18 vom: 29. Sept., Seite 5532-5546
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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| 773 |
1 |
8 |
|g volume:74
|g year:2023
|g number:18
|g day:29
|g month:09
|g pages:5532-5546
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| 856 |
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|u http://dx.doi.org/10.1093/jxb/erad255
|3 Volltext
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