Shaping the phycosphere : Analysis of the EPS in diatom-bacterial co-cultures

© 2023 The Authors. Journal of Phycology published by Wiley Periodicals LLC on behalf of Phycological Society of America.

Bibliographische Detailangaben
Veröffentlicht in:Journal of phycology. - 1966. - 59(2023), 4 vom: 12. Aug., Seite 791-797
1. Verfasser: Daly, Giulia (VerfasserIn)
Weitere Verfasser: Decorosi, Francesca, Viti, Carlo, Adessi, Alessandra
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Journal of phycology
Schlagworte:Journal Article Research Support, Non-U.S. Gov't dual-system co-culture exopolysaccharides extracellular environment monosaccharide composition phycosphere Polymers
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520 |a The phycosphere is a unique niche that fosters complex interactions between microalgae and associated bacteria. The formation of this extracellular environment, and the associated bacterial biodiversity, is heavily influenced by the secretion of extracellular polymers, primarily driven by phototrophic organisms. The exopolysaccharides (EPS) represent the largest fraction of the microalgae-derived exudates, which can be specifically used by heterotrophic bacteria as substrates for metabolic processes. Furthermore, it has been proposed that bacteria and their extracellular factors play a role in both the release and composition of the EPS. In this study, two model microorganisms, the diatom Phaeodactylum tricornutum CCAP 1055/15 and the bacterium Pseudoalteromonas haloplanktis TAC125, were co-cultured in a dual system to assess how their interactions modify the phycosphere chemical composition by analyzing the EPS monosaccharide profile released in the culture media by the two partners. We demonstrate that microalgal-bacterial interactions in this simplified model significantly influenced the architecture of their extracellular environment. We observed that the composition of the exo-environment, as described by the EPS monosaccharide profiles, varied under different culture conditions and times of incubation. This study reports an initial characterization of the molecular modifications occurring in the extracellular environment surrounding two relevant representatives of marine systems 
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650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a dual-system co-culture 
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650 4 |a phycosphere 
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700 1 |a Decorosi, Francesca  |e verfasserin  |4 aut 
700 1 |a Viti, Carlo  |e verfasserin  |4 aut 
700 1 |a Adessi, Alessandra  |e verfasserin  |4 aut 
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