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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2023.154047
|2 doi
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|a pubmed24n1196.xml
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|a (DE-627)NLM358951151
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|a (NLM)37393886
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|a (PII)S0176-1617(23)00141-4
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|a DE-627
|b ger
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|e rakwb
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|a eng
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|a Talla, Sai Krishna
|e verfasserin
|4 aut
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|a Redox basis of photosynthesis inhibition at supra-optimal bicarbonate in mesophyll protoplasts of Arabidopsis thaliana
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|c 2023
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|a Text
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|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 14.08.2023
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|a Date Revised 14.08.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier GmbH. All rights reserved.
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|a We examined the patterns of photosynthetic O2 evolution at 1 mM (optimal) and 10 mM (supra-optimal) bicarbonate in mesophyll protoplasts of Arabidopsis thaliana. The photosynthetic rate of protoplasts reached the maximum at an optimal concentration of 1 mM bicarbonate and got suppressed at supra-optimal levels of bicarbonate. We examined the basis of such photosynthesis inhibition by mesophyll protoplasts at supra-optimal bicarbonate. The wild-type protoplasts exposed to supra-optimal bicarbonate showed up signs of oxidative stress. Besides the wild-type, two mutants were used: nadp-mdh (deficient in chloroplastic NADP-MDH) and vtc1 (deficient in mitochondrial ascorbate biosynthesis). The protoplasts of the nadp-mdh mutant exhibited a higher photosynthetic rate and greater sensitivity to supra-optimal bicarbonate than the wild-type. The ascorbate-deficient vtc1 mutant had a low photosynthetic rate and no significant inhibition at high bicarbonate. The nadp-mdh mutants had elevated activities, protein, and transcript levels of key antioxidant enzymes. On the other hand, the antioxidant enzyme systems in vtc1 mutants were not much affected at supra-optimal bicarbonate. We propose that the inhibition of photosynthesis at supra-optimal bicarbonate depends on the redox state of mesophyll protoplasts. The robust antioxidant enzyme systems in protoplasts of nadp-mdh mutant might be priming the plants to sustain high photosynthesis at supra-optimal bicarbonate
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|a Journal Article
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|a Antioxidant defense systems
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|a Arabidopsis
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|a Bicarbonate
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|a Redox status
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|a nadp-mdh mutant
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|a vtc1 mutant
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|a Antioxidants
|2 NLM
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|a Bicarbonates
|2 NLM
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|a NADP
|2 NLM
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|a 53-59-8
|2 NLM
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|a Ascorbic Acid
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|a PQ6CK8PD0R
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|a Arabidopsis Proteins
|2 NLM
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|a Sunil, Bobba
|e verfasserin
|4 aut
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|a Rao, Duvvarapu Easwar
|e verfasserin
|4 aut
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|a Rajsheel, Pidakala
|e verfasserin
|4 aut
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|a Saini, Deepak
|e verfasserin
|4 aut
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|a Raghavendra, Agepati S
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 287(2023) vom: 15. Aug., Seite 154047
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnns
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| 773 |
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|g volume:287
|g year:2023
|g day:15
|g month:08
|g pages:154047
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|u http://dx.doi.org/10.1016/j.jplph.2023.154047
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