The Medicago truncatula nodule-specific cysteine-rich peptides, NCR343 and NCR-new35 are required for the maintenance of rhizobia in nitrogen-fixing nodules

The Medicago truncatula nodule-specific cysteine-rich peptides, NCR343 and NCR-new35 are required for the maintenance of rhizobia in nitrogen-fixing nodules

© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 239(2023), 5 vom: 13. Sept., Seite 1974-1988
1. Verfasser: Horváth, Beatrix (VerfasserIn)
Weitere Verfasser: Güngör, Berivan, Tóth, Mónika, Domonkos, Ágota, Ayaydin, Ferhan, Saifi, Farheen, Chen, Yuhui, Biró, János Barnabás, Bourge, Mickael, Szabó, Zoltán, Tóth, Zoltán, Chen, Rujin, Kaló, Péter
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Medicago truncatula indeterminate nodule legume nitrogen-fixing symbiosis nodule-specific cysteine-rich peptide Cysteine K848JZ4886 Nitrogen mehr... N762921K75 Peptides
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520 |a In the nodules of IRLC legumes, including Medicago truncatula, nitrogen-fixing rhizobia undergo terminal differentiation resulting in elongated and endoreduplicated bacteroids specialized for nitrogen fixation. This irreversible transition of rhizobia is mediated by host produced nodule-specific cysteine-rich (NCR) peptides, of which c. 700 are encoded in the M. truncatula genome but only few of them have been proved to be essential for nitrogen fixation. We carried out the characterization of the nodulation phenotype of three ineffective nitrogen-fixing M. truncatula mutants using confocal and electron microscopy, monitored the expression of defence and senescence-related marker genes, and analysed the bacteroid differentiation with flow cytometry. Genetic mapping combined with microarray- or transcriptome-based cloning was used to identify the impaired genes. Mtsym19 and Mtsym20 mutants are defective in the same peptide NCR-new35 and the lack of NCR343 is responsible for the ineffective symbiosis of NF-FN9363. We found that the expression of NCR-new35 is significantly lower and limited to the transition zone of the nodule compared with other crucial NCRs. The fluorescent protein-tagged version of NCR343 and NCR-new35 localized to the symbiotic compartment. Our discovery added two additional members to the group of NCR genes essential for nitrogen-fixing symbiosis in M. truncatula 
650 4 |a Journal Article 
650 4 |a Research Support, Non-U.S. Gov't 
650 4 |a Medicago truncatula 
650 4 |a indeterminate nodule 
650 4 |a legume 
650 4 |a nitrogen-fixing symbiosis 
650 4 |a nodule-specific cysteine-rich peptide 
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650 7 |a Nitrogen  |2 NLM 
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700 1 |a Güngör, Berivan  |e verfasserin  |4 aut 
700 1 |a Tóth, Mónika  |e verfasserin  |4 aut 
700 1 |a Domonkos, Ágota  |e verfasserin  |4 aut 
700 1 |a Ayaydin, Ferhan  |e verfasserin  |4 aut 
700 1 |a Saifi, Farheen  |e verfasserin  |4 aut 
700 1 |a Chen, Yuhui  |e verfasserin  |4 aut 
700 1 |a Biró, János Barnabás  |e verfasserin  |4 aut 
700 1 |a Bourge, Mickael  |e verfasserin  |4 aut 
700 1 |a Szabó, Zoltán  |e verfasserin  |4 aut 
700 1 |a Tóth, Zoltán  |e verfasserin  |4 aut 
700 1 |a Chen, Rujin  |e verfasserin  |4 aut 
700 1 |a Kaló, Péter  |e verfasserin  |4 aut 
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773 1 8 |g volume:239  |g year:2023  |g number:5  |g day:13  |g month:09  |g pages:1974-1988 
856 4 0 |u http://dx.doi.org/10.1111/nph.19097  |3 Volltext 
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