Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes

Biocatalytic Foams from Microdroplet-Formulated Self-Assembling Enzymes

© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.

Détails bibliographiques
Publié dans:Advanced materials (Deerfield Beach, Fla.). - 1998. - 35(2023), 39 vom: 26. Sept., Seite e2303952
Auteur principal: Hertel, Julian S (Auteur)
Autres auteurs: Bitterwolf, Patrick, Kröll, Sandra, Winterhalter, Astrid, Weber, Annika J, Grösche, Maximilian, Walkowsky, Laurenz B, Heißler, Stefan, Schwotzer, Matthias, Wöll, Christof, van de Kamp, Thomas, Zuber, Marcus, Baumbach, Tilo, Rabe, Kersten S, Niemeyer, Christof M
Format: Article en ligne
Langue:English
Publié: 2023
Accès à la collection:Advanced materials (Deerfield Beach, Fla.)
Sujets:Journal Article enzymes flow biocatalysis microreactors monodisperse foams porous materials Alcohols Enzymes, Immobilized Enzymes
Description
Résumé:© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
Industrial biocatalysis plays an important role in the development of a sustainable economy, as enzymes can be used to synthesize an enormous range of complex molecules under environmentally friendly conditions. To further develop the field, intensive research is being conducted on process technologies for continuous flow biocatalysis in order to immobilize large quantities of enzyme biocatalysts in microstructured flow reactors under conditions that are as gentle as possible in order to realize efficient material conversions. Here, monodisperse foams consisting almost entirely of enzymes covalently linked via SpyCatcher/SpyTag conjugation are reported. The biocatalytic foams are readily available from recombinant enzymes via microfluidic air-in-water droplet formation, can be directly integrated into microreactors, and can be used for biocatalytic conversions after drying. Reactors prepared by this method show surprisingly high stability and biocatalytic activity. The physicochemical characterization of the new materials is described and exemplary applications in biocatalysis are shown using two-enzyme cascades for the stereoselective synthesis of chiral alcohols and the rare sugar tagatose
Description:Date Completed 28.09.2023
Date Revised 28.09.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.202303952