Ptr1 and ZAR1 immune receptors confer overlapping and distinct bacterial pathogen effector specificities

Bibliographische Detailangaben
Veröffentlicht in:	The New phytologist. - 1979. - 239(2023), 5 vom: 05. Sept., Seite 1935-1953
1. Verfasser:	Ahn, Ye Jin (VerfasserIn)
Weitere Verfasser:	Kim, Haseong, Choi, Sera, Mazo-Molina, Carolina, Prokchorchik, Maxim, Zhang, Ning, Kim, Boyoung, Mang, Hyunggon, Koehler, Naio, Kim, Jieun, Lee, Soeui, Yoon, Hayeon, Choi, Doil, Kim, Min-Sung, Segonzac, Cécile, Martin, Gregory B, Schultink, Alex, Sohn, Kee Hoon
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2023
Zugriff auf das übergeordnete Werk:	The New phytologist
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Nicotiana benthamiana NLR convergent evolution effector recognition plant immune receptors Proteins Carrier Proteins mehr... Receptors, Immunologic Bacterial Proteins Arabidopsis Proteins RIN4 protein, Arabidopsis Intracellular Signaling Peptides and Proteins ZAR1 protein, Arabidopsis

Beschreibung
Zusammenfassung:	© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation. Some nucleotide-binding and leucine-rich repeat receptors (NLRs) indirectly detect pathogen effectors by monitoring their host targets. In Arabidopsis thaliana, RIN4 is targeted by multiple sequence-unrelated effectors and activates immune responses mediated by RPM1 and RPS2. These effectors trigger cell death in Nicotiana benthamiana, but the corresponding NLRs have yet not been identified. To identify N. benthamiana NLRs (NbNLRs) that recognize Arabidopsis RIN4-targeting effectors, we conducted a rapid reverse genetic screen using an NbNLR VIGS library. We identified that the N. benthamiana homolog of Ptr1 (Pseudomonas tomato race 1) recognizes the Pseudomonas effectors AvrRpt2, AvrRpm1, and AvrB. We demonstrated that recognition of the Xanthomonas effector AvrBsT and the Pseudomonas effector HopZ5 is conferred independently by the N. benthamiana homolog of Ptr1 and ZAR1. Interestingly, the recognition of HopZ5 and AvrBsT is contributed unequally by Ptr1 and ZAR1 in N. benthamiana and Capsicum annuum. In addition, we showed that the RLCK XII family protein JIM2 is required for the NbZAR1-dependent recognition of AvrBsT and HopZ5. The recognition of sequence-unrelated effectors by NbPtr1 and NbZAR1 provides an additional example of convergently evolved effector recognition. Identification of key components involved in Ptr1 and ZAR1-mediated immunity could reveal unique mechanisms of expanded effector recognition
Beschreibung:	Date Completed 03.08.2023 Date Revised 15.02.2024 published: Print-Electronic RefSeq: MH532570 ErratumIn: New Phytol. 2024 Feb 15;:. - PMID 38357992 Citation Status MEDLINE
ISSN:	1469-8137
DOI:	10.1111/nph.19073