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|a 10.1094/PDIS-04-23-0706-RE
|2 doi
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|a DE-627
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|a eng
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| 100 |
1 |
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|a Heck, Daniel W
|e verfasserin
|4 aut
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| 245 |
1 |
0 |
|a Enabling Population Biology Studies of Stemphylium vesicarium from Onion with Microsatellites
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| 264 |
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|c 2023
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| 336 |
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|a Text
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|a ƒaComputermedien
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|a Date Completed 01.01.2024
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|a Date Revised 02.01.2024
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Stemphylium leaf blight (SLB), caused by the fungus Stemphylium vesicarium, is dominant within the foliar disease complex affecting onion production in New York (NY). The disease causes premature defoliation and significant reductions in bulb weight and quality. Foliar diseases of onion are usually managed by an intensive fungicide program, but SLB management is complicated by resistance to multiple single-site modes of action. The design of integrated disease management strategies is limited by incomplete knowledge surrounding the dominant sources of S. vesicarium inoculum. To facilitate genomic-based studies of S. vesicarium populations, nine microsatellite markers were developed. The markers were multiplexed into two PCR assays containing four and five fluorescently labeled microsatellite markers. Initial testing of the S. vesicarium isolates found the markers were highly polymorphic and reproducible with an average of 8.2 alleles per locus. The markers were used to characterize 54 S. vesicarium isolates from major NY onion production regions in 2016 (n = 27) and 2018 (n = 27). Fifty-two multilocus genotypes (MLGs) were identified between these populations. Genotypic and allelic diversities were high in both the 2016 and 2018 populations. A greater degree of genetic variation was observed within populations than between years. No distinct pattern of MLGs according to population was identified and some MLGs were closely related between 2016 and 2018. The lack of evidence for linkage among loci also was strongly suggestive of clonal populations with only minor differences between the two populations. These microsatellite markers will be a foundational resource for the testing of hypotheses surrounding the population biology of S. vesicarium and therefore informing disease management
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4 |
|a Journal Article
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| 650 |
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4 |
|a Allium cepa
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| 650 |
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4 |
|a SSR
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| 650 |
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4 |
|a Stemphylium leaf blight
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| 650 |
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4 |
|a epidemiology
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| 650 |
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4 |
|a genetic diversity
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| 650 |
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4 |
|a population genetics
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| 700 |
1 |
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|a Hay, Frank
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Pethybridge, Sarah J
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Plant disease
|d 1997
|g 107(2023), 12 vom: 01. Dez., Seite 3886-3895
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|x 0191-2917
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|g volume:107
|g year:2023
|g number:12
|g day:01
|g month:12
|g pages:3886-3895
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| 856 |
4 |
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|u http://dx.doi.org/10.1094/PDIS-04-23-0706-RE
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