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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.107829
|2 doi
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|a pubmed25n1194.xml
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|a (DE-627)NLM358312523
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|a (NLM)37329690
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|a (PII)S0981-9428(23)00340-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Ali, Muhammad Moaaz
|e verfasserin
|4 aut
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|a Exogenously applied zinc improves sugar-acid profile of loquat (Eriobotrya japonica Lindl.) by regulating enzymatic activities and expression of their metabolism-related genes
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 12.08.2023
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|a published: Print-Electronic
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|a Citation Status Publisher
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|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
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|a Soluble sugars and organic acids are the most abundant components in ripe fruits, and they play critical roles in the development of fruit flavor and taste. In this study, loquat trees were sprayed with 0.1, 0.2 and 0.3% zinc sulphate. The contents of soluble sugars and organic acids were determined using HPLC-RID and UPLC-MS, respectively. The activities of key enzymes involved in sugar-acid metabolism were measured and expression profiling of related genes was done using RT-qPCR. The results revealed that 0.1% zinc sulphate was a promising treatment among other Zn applications with respect to the increased levels of soluble sugars and decreased acid contents in loquats. Correlation analysis showed that the enzymes i.e., SPS, SS, FK, and HK were may be involved in the regulation of fructose and glucose metabolism in the fruit pulp of loquat. While, the activity of NADP-ME showed negative and NAD-MDH showed a positive correlation with malic acid content. Meanwhile, EjSPS1-4, EjSS2-4, EjHK1-3, and EjFK1-6 may play an important role in soluble sugar metabolism in the pulp of loquat fruits. Similarly, EjPEPC2, EjPEPC3, EjNAD-MDH1, EjNAD-MDH3-5, EjNAD-MDH6 and EjNAD-MDH13 may have a vital contribution to malic acid biosynthesis in loquat fruits. This study provides new insights for future elucidation of key mechanisms regulating soluble sugars and malic acid biosynthesis in loquats
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|a Journal Article
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|a Fructokinase
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|a Fructose
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|a HPLC
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|a Malate dehydrogenase
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|a Malic acid
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|a Zinc sulphate
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|a Gull, Shaista
|e verfasserin
|4 aut
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|a Hu, Xiaobo
|e verfasserin
|4 aut
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|a Hou, Youming
|e verfasserin
|4 aut
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|a Chen, Faxing
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 201(2023) vom: 15. Aug., Seite 107829
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
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|g volume:201
|g year:2023
|g day:15
|g month:08
|g pages:107829
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|u http://dx.doi.org/10.1016/j.plaphy.2023.107829
|3 Volltext
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