Overexpression of potato ORANGE (StOR) and StOR mutant in Arabidopsis confers increased carotenoid accumulation and tolerance to abiotic stress

Overexpression of potato ORANGE (StOR) and StOR mutant in Arabidopsis confers increased carotenoid accumulation and tolerance to abiotic stress

Copyright © 2023 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 201(2023) vom: 14. Aug., Seite 107809
1. Verfasser: Kang, Le (VerfasserIn)
Weitere Verfasser: Zhang, Chunli, Liu, Junke, Ye, Muying, Zhang, Li, Chen, Fengfeng, Lin, Xinyue, Yang, Dongjing, Ren, Liping, Li, Yunxiang, Kim, Ho Soo, Kwak, Sang-Soo, Li, Hongbing, Deng, Xiping, Zhang, Peng, Ke, Qingbo
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Abiotic stress Carotenoid ORANGE gene ORANGE mutant Potato beta Carotene 01YAE03M7J Carotenoids 36-88-4 Plant Proteins
Beschreibung
Zusammenfassung:Copyright © 2023 Elsevier Masson SAS. All rights reserved.
ORANGE (OR) plays essential roles in regulating carotenoid homeostasis and enhancing the ability of plants to adapt to environmental stress. However, OR proteins have been functionally characterized in only a few plant species, and little is known about the role of potato OR (StOR). In this study, we characterized the StOR gene in potato (Solanum tuberosum L. cv. Atlantic). StOR is predominantly localized to the chloroplast, and its transcripts are tissue-specifically expressed and significantly induced in response to abiotic stress. Compared with wild type, overexpression of StOR increased β-carotene levels up to 4.8-fold, whereas overexpression of StORHis with a conserved arginine to histidine substitution promoted β-carotene accumulation up to 17.6-fold in Arabidopsis thaliana calli. Neither StOR nor StORHis overexpression dramatically affected the transcript levels of carotenoid biosynthetic genes. Furthermore, overexpression of either StOR or StORHis increased abiotic stress tolerance in Arabidopsis, which was associated with higher photosynthetic capacity and antioxidative activity. Taken together, these results indicate that StOR could be exploited as a potential new genetic tool for the improvement of crop nutritional quality and environmental stress tolerance
Beschreibung:Date Completed 14.08.2023
Date Revised 14.08.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1873-2690
DOI:10.1016/j.plaphy.2023.107809