Multi-omics reveal key enzymes involved in the formation of phenylpropanoid glucosides in Artemisia annua

Multi-omics reveal key enzymes involved in the formation of phenylpropanoid glucosides in Artemisia annua

Copyright © 2023 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 201(2023) vom: 01. Aug., Seite 107795
1. Verfasser: Yin, Qinggang (VerfasserIn)
Weitere Verfasser: Wu, Tianze, Gao, Ranran, Wu, Lan, Shi, Yuhua, Wang, Xingwen, Wang, Mengyue, Xu, Zhichao, Zhao, Yueliang, Su, Xiaojia, Su, Yanyan, Han, Xiaoyan, Yuan, Ling, Xiang, Li, Chen, Shilin
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article AlphaFold Artemisia annua Glycosyltransferase Methyltransferase Phenylpropanoid Scopolin Scopoletin KLF1HS0SXJ Esculin mehr... 1Y1L18LQAF artemisinin 9RMU91N5K2 Artemisinins Glucosides Glucose IY9XDZ35W2 Uridine Diphosphate 58-98-0
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100 1 |a Yin, Qinggang  |e verfasserin  |4 aut 
245 1 0 |a Multi-omics reveal key enzymes involved in the formation of phenylpropanoid glucosides in Artemisia annua 
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500 |a Date Completed 14.08.2023 
500 |a Date Revised 14.08.2023 
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500 |a Citation Status MEDLINE 
520 |a Copyright © 2023 Elsevier Masson SAS. All rights reserved. 
520 |a Although mainly known for producing artemisinin, Artemisia annua is enriched in phenylpropanoid glucosides (PGs) with significant bioactivities. However, the biosynthesis of A. annua PGs is insufficiently investigated. Different A. annua ecotypes from distinct growing environments accumulate varying amounts of metabolites, including artemisinin and PGs such as scopolin. UDP-glucose:phenylpropanoid glucosyltransferases (UGTs) transfers glucose from UDP-glucose in PG biosynthesis. Here, we found that the low-artemisinin ecotype GS produces a higher amount of scopolin, compared to the high-artemisinin ecotype HN. By combining transcriptome and proteome analyses, we selected 28 candidate AaUGTs from 177 annotated AaUGTs. Using AlphaFold structural prediction and molecular docking, we determined the binding affinities of 16 AaUGTs. Seven of the AaUGTs enzymatically glycosylated phenylpropanoids. AaUGT25 converted scopoletin to scopolin and esculetin to esculin. The lack of accumulation of esculin in the leaf and the high catalytic efficiency of AaUGT25 on esculetin suggest that esculetin is methylated to scopoletin, the precursor of scopolin. We also discovered that AaOMT1, a previously uncharacterized O-methyltransferase, converts esculetin to scopoletin, suggesting an alternative route for producing scopoletin, which contributes to the high-level accumulation of scopolin in A. annua leaves. AaUGT1 and AaUGT25 responded to induction of stress-related phytohormones, implying the involvement of PGs in stress responses 
650 4 |a Journal Article 
650 4 |a AlphaFold 
650 4 |a Artemisia annua 
650 4 |a Glycosyltransferase 
650 4 |a Methyltransferase 
650 4 |a Phenylpropanoid 
650 4 |a Scopolin 
650 7 |a Scopoletin  |2 NLM 
650 7 |a KLF1HS0SXJ  |2 NLM 
650 7 |a Esculin  |2 NLM 
650 7 |a 1Y1L18LQAF  |2 NLM 
650 7 |a artemisinin  |2 NLM 
650 7 |a 9RMU91N5K2  |2 NLM 
650 7 |a Artemisinins  |2 NLM 
650 7 |a Glucosides  |2 NLM 
650 7 |a Glucose  |2 NLM 
650 7 |a IY9XDZ35W2  |2 NLM 
650 7 |a Uridine Diphosphate  |2 NLM 
650 7 |a 58-98-0  |2 NLM 
700 1 |a Wu, Tianze  |e verfasserin  |4 aut 
700 1 |a Gao, Ranran  |e verfasserin  |4 aut 
700 1 |a Wu, Lan  |e verfasserin  |4 aut 
700 1 |a Shi, Yuhua  |e verfasserin  |4 aut 
700 1 |a Wang, Xingwen  |e verfasserin  |4 aut 
700 1 |a Wang, Mengyue  |e verfasserin  |4 aut 
700 1 |a Xu, Zhichao  |e verfasserin  |4 aut 
700 1 |a Zhao, Yueliang  |e verfasserin  |4 aut 
700 1 |a Su, Xiaojia  |e verfasserin  |4 aut 
700 1 |a Su, Yanyan  |e verfasserin  |4 aut 
700 1 |a Han, Xiaoyan  |e verfasserin  |4 aut 
700 1 |a Yuan, Ling  |e verfasserin  |4 aut 
700 1 |a Xiang, Li  |e verfasserin  |4 aut 
700 1 |a Chen, Shilin  |e verfasserin  |4 aut 
773 0 8 |i Enthalten in  |t Plant physiology and biochemistry : PPB  |d 1991  |g 201(2023) vom: 01. Aug., Seite 107795  |w (DE-627)NLM098178261  |x 1873-2690  |7 nnns 
773 1 8 |g volume:201  |g year:2023  |g day:01  |g month:08  |g pages:107795 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2023.107795  |3 Volltext 
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