Identification of a Prunus MAX1 homolog as a unique strigol synthase
© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
| Veröffentlicht in: | The New phytologist. - 1979. - 239(2023), 5 vom: 15. Sept., Seite 1819-1833 |
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| 1. Verfasser: | |
| Weitere Verfasser: | , , , , , , , |
| Format: | Online-Aufsatz |
| Sprache: | English |
| Veröffentlicht: |
2023
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| Zugriff auf das übergeordnete Werk: | The New phytologist |
| Schlagworte: | Journal Article Research Support, Non-U.S. Gov't CYP711A MAX1 biosynthesis cytochrome P450 peach (Prunus persica) strigolactone strigol 7I81Q4NS29 mehr... |
| Zusammenfassung: | © 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation. Strigol is the first identified and one of the most important strigolactones (SLs), but the biosynthetic pathway remains elusive. We functionally identified a strigol synthase (cytochrome P450 711A enzyme) in the Prunus genus through rapid gene screening in a set of SL-producing microbial consortia, and confirmed its unique catalytic activity (catalyzing multistep oxidation) through substrate feeding experiments and mutant analysis. We also reconstructed the biosynthetic pathway of strigol in Nicotiana benthamiana and reported the total biosynthesis of strigol in the Escherichia coli-yeast consortium, from the simple sugar xylose, which paves the way for large-scale production of strigol. As proof of concept, strigol and orobanchol were detected in Prunus persica root extrudes. This demonstrated a successful prediction of metabolites produced in plants through gene function identification, highlighting the importance of deciphering the sequence-function correlation of plant biosynthetic enzymes to more accurately predicate plant metabolites without metabolic analysis. This finding revealed the evolutionary and functional diversity of CYP711A (MAX1) in SL biosynthesis, which can synthesize different stereo-configurations of SLs (strigol- or orobanchol-type). This work again emphasizes the importance of microbial bioproduction platform as an efficient and handy tool to functionally identify plant metabolism |
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| Beschreibung: | Date Completed 03.08.2023 Date Revised 03.08.2023 published: Print-Electronic RefSeq: NP_565617, XP_007225050, XP_007224581, XP_007222310, XP_027918387, XP_016745621, XP_020424098, XP_015633367, XP_015644699, BCG55903, XP_002458367, KAG0530922, XP_002443327, XP_007222310.1, XP_007224581.1, XP_007225050.2, XP_008393629, XP_028955031, XP_008357300, RXH72971 Citation Status MEDLINE |
| ISSN: | 1469-8137 |
| DOI: | 10.1111/nph.19052 |