Site-specific analysis of ribosomal 2'O-methylation by quantitative reverse transcription PCR under low deoxynucleotide triphosphate concentrations

Ribose 2'O-methylation (Nm, ribomethylation) is the most abundant RNA modification present in rRNA. It has been shown that alterations in ribosomal 2'O-methylation at individual Nm sites likely reflect regulated cellular processes. Although several analytical approaches for Nm detection an...

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Veröffentlicht in:	BioTechniques. - 1991. - 74(2023), 5 vom: 15. Mai, Seite 225-235
1. Verfasser:	Barros-Silva, Daniela (VerfasserIn)
Weitere Verfasser:	Tsui, Johan, Jerónimo, Carmen, Jenster, Guido, Martens-Uzunova, Elena S
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2023
Zugriff auf das übergeordnete Werk:	BioTechniques
Schlagworte:	Journal Article Research Support, Non-U.S. Gov't 2′O-methylation C/D-box snoRNA epitranscriptomics quantitative real-time PCR ribosomal RNA Methyltransferases EC 2.1.1.- triphosphoric acid mehr... NU43IAG5BC RNA 63231-63-0 RNA, Ribosomal