Nucleoredoxin gene SINRX1 negatively regulates tomato immunity by activating SA signaling pathway

Bibliographische Detailangaben
Veröffentlicht in:	Plant physiology and biochemistry : PPB. - 1991. - 200(2023) vom: 15. Juli, Seite 107804
1. Verfasser:	Cha, Joon Yung (VerfasserIn)
Weitere Verfasser:	Uddin, Shahab, Macoy, Donah Mary, Shin, Gyeong-Im, Jeong, Song Yi, Ali, Imdad, Hwang, Ji-Won, Ji, Myung Geun, Lee, Sang Cheol, Park, Joung Hun, Sultana, Marium, Ryu, Gyeong Ryul, Ahn, Gyeongik, Lee, Sang Yeol, Kim, Min Gab, Kim, Woe-Yeon
Format:	Online-Aufsatz
Sprache:	English
Veröffentlicht:	2023
Zugriff auf das übergeordnete Werk:	Plant physiology and biochemistry : PPB
Schlagworte:	Journal Article Jasmonic acid Pathogen Salicylic acid SlNRX1 Tomato Salicylic Acid O414PZ4LPZ nucleoredoxin EC 1.- Cyclopentanes

Beschreibung
Zusammenfassung:	Copyright © 2023 Elsevier Masson SAS. All rights reserved. The tomato (Solanum lycopersicum) is widely consumed globally and renowned for its health benefits, including the reduction of cardiovascular disease and prostate cancer risk. However, tomato production faces significant challenges, particularly due to various biotic stresses such as fungi, bacteria, and viruses. To address this challenges, we employed the CRISPR/Cas9 system to modify the tomato NUCLEOREDOXIN (SlNRX) genes (SlNRX1 and SlNRX2) belonging to the nucleocytoplasmic THIOREDOXIN subfamily. CRISPR/Cas9-mediated mutations in SlNRX1 (slnrx1) plants exhibited resistance against bacterial leaf pathogen Pseudomonas syringae pv. maculicola (Psm) ES4326, as well as the fungal pathogen Alternaria brassicicola. However, the slnrx2 plants did not display resistance. Notably, the slnrx1 demonstrated elevated levels of endogenous salicylic acid (SA) and reduced levels of jasmonic acid after Psm infection, in comparison to both wild-type (WT) and slnrx2 plants. Furthermore, transcriptional analysis revealed that genes involved in SA biosynthesis, such as ISOCHORISMATE SYNTHASE 1 (SlICS1) and ENHANCED DISEASE SUSCEPTIBILITY 5 (SlEDS5), were upregulated in slnrx1 compared to WT plants. In addition, a key regulator of systemic acquired resistance, PATHOGENESIS-RELATED 1 (PR1), exhibited increased expression in slnrx1 compared to WT. These findings suggest that SlNRX1 acts as a negative regulator of plant immunity, facilitating infection by the Psm pathogen through interference with the phytohormone SA signaling pathway. Thus, targeted mutagenesis of SlNRX1 is a promising genetic means to enhance biotic stress resistance in crop breeding
Beschreibung:	Date Completed 12.06.2023 Date Revised 12.06.2023 published: Print-Electronic Citation Status MEDLINE
ISSN:	1873-2690
DOI:	10.1016/j.plaphy.2023.107804