Gibson assembly interposition improves amplification efficiency of long DNA and multifragment overlap extension PCR
For difficult overlap extension PCR, a Gibson assembly process was inserted between the two PCR rounds to facilitate the formation of complete gene templates at a moderate temperature. That is, after amplifying each DNA fragment, they were preluded by a Gibson assembly process in equal proportion. T...
Ausführliche Beschreibung
Bibliographische Detailangaben
| Veröffentlicht in: | BioTechniques. - 1993. - 74(2023), 6 vom: 31. Juni, Seite 286-292
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| 1. Verfasser: |
Liu, Junyi
(VerfasserIn) |
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| Weitere Verfasser: |
Liu, Fangyin,
Luo, Xueer,
Chen, Ming,
Wang, Chengjun,
Wang, Liuyue,
Chen, Huabo |
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| Format: | Online-Aufsatz
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| Sprache: | English |
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| Veröffentlicht: |
2023
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| Zugriff auf das übergeordnete Werk: | BioTechniques
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| Schlagworte: | Journal Article
Research Support, Non-U.S. Gov't
Gibson assembly
gene fusion
overlap extension PCR
retinoblastoma gene
site-directed mutagenesis
DNA
9007-49-2 |
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