Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development

Essential role of the CD docking motif of MPK4 in plant immunity, growth, and development

© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 239(2023), 3 vom: 25. Aug., Seite 1112-1126
1. Verfasser: Siodmak, Anna (VerfasserIn)
Weitere Verfasser: Shahul Hameed, Umar F, Rayapuram, Naganand, Völz, Ronny, Boudsocq, Marie, Alharbi, Siba, Alhoraibi, Hannah, Lee, Yong-Hwan, Blilou, Ikram, Arold, Stefan T, Hirt, Heribert
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't MPK4 immunity kinase activity oxidation reactive oxygen species Mitogen-Activated Protein Kinases EC 2.7.11.24 Arabidopsis Proteins mehr... Mitogen-Activated Protein Kinase Kinases EC 2.7.12.2
Beschreibung
Zusammenfassung:© 2023 The Authors. New Phytologist © 2023 New Phytologist Foundation.
MAPKs are universal eukaryotic signaling factors whose functioning is assumed to depend on the recognition of a common docking motif (CD) by its activators, substrates, and inactivators. We studied the role of the CD domain of Arabidopsis MPK4 by performing interaction studies and determining the ligand-bound MPK4 crystal structure. We revealed that the CD domain of MPK4 is essential for interaction and activation by its upstream MAPKKs MKK1, MKK2, and MKK6. Cys181 in the CD site of MPK4 was shown to become sulfenylated in response to reactive oxygen species in vitro. To test the function of C181 in vivo, we generated wild-type (WT) MPK4-C181, nonsulfenylatable MPK4-C181S, and potentially sulfenylation mimicking MPK4-C181D lines in the mpk4 knockout background. We analyzed the phenotypes in growth, development, and stress responses, revealing that MPK4-C181S has WT activity and complements the mpk4 phenotype. By contrast, MPK4-C181D cannot be activated by upstream MAPKK and cannot complement the phenotypes of mpk4. Our findings show that the CD motif is essential and is required for activation by upstream MAPKK for MPK4 function. Furthermore, growth, development, or immunity functions require upstream activation of the MPK4 protein kinase
Beschreibung:Date Completed 30.06.2023
Date Revised 03.10.2023
published: Print-Electronic
ErratumIn: New Phytol. 2023 Dec;240(5):2164. - PMID 37787053
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18989