CBL1/9-CIPK23-NRAMP1 axis regulates manganese toxicity

CBL1/9-CIPK23-NRAMP1 axis regulates manganese toxicity

© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.

Bibliographische Detailangaben
Veröffentlicht in:The New phytologist. - 1979. - 239(2023), 2 vom: 01. Juli, Seite 660-672
1. Verfasser: Zhang, Zhenqian (VerfasserIn)
Weitere Verfasser: Fu, Dali, Xie, Dixiang, Wang, Zhangqing, Zhao, Yuqing, Ma, Xuening, Huang, Panpan, Ju, Chuanfeng, Wang, Cun
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:The New phytologist
Schlagworte:Journal Article Research Support, Non-U.S. Gov't CBL-interacting protein kinases (CIPKs) Ca2+ Mn transporter NRAMP1 calcineurin B-like proteins (CBLs) manganese Arabidopsis Proteins Calcium-Binding Proteins CIPK23 protein, Arabidopsis mehr... EC 2.7.11.1 Manganese 42Z2K6ZL8P Protein Serine-Threonine Kinases NRAMP1 protein, Arabidopsis calcineurin B-like protein 1, Arabidopsis CBL9 protein, Arabidopsis
Beschreibung
Zusammenfassung:© 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
Manganese (Mn) is an essential micronutrient in plants. However, excessive Mn absorption in acidic soils can cause Mn toxicity, which adversely affects plant growth and crop yields. At present, acidic soils cover c. 30% of the Earth's surface. However, the mechanism underpinning Mn uptake remains largely unknown. We identified cbl1/9 and cipk23 mutants exhibiting high-Mn-sensitive phenotype through the reverse genetics method. Furthermore, we identified the CIPK23 phosphorylated NRAMP1 through a variety of protein interaction techniques and protein kinase assays. Here, we demonstrated that two calcineurin B-like proteins, CBL1/9, and their interacting kinase CIPK23 positively regulated the tolerance of Mn toxicity in Arabidopsis. The cbl1 cbl9 double mutant and cipk23 mutants exhibited high-Mn-sensitive phenotypes, which manifested as decreased primary root length, biomass, and chlorophyll concentration, and higher accumulation of Mn. In addition, CIPK23 interacted with and phosphorylated the Mn transporter NRAMP1 primarily at Ser20/22 in vitro and in vivo, and thereby induced clathrin-mediated endocytosis of NRAMP1 to reduce its distribution on the plasma membrane and enhance plant tolerance to Mn toxicity. In summary, we found that the CBL1/9-CIPK23-NRAMP1 module regulates the tolerance to high-Mn toxicity and provide insight into a mechanism of the tolerance of plants to Mn toxicity
Beschreibung:Date Completed 22.06.2023
Date Revised 22.06.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1469-8137
DOI:10.1111/nph.18968