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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1007/s11240-023-02495-6
|2 doi
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|a eng
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|a Ferreira, Ana Clara
|e verfasserin
|4 aut
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|a A simplified protocol for Agrobacterium-mediated transformation of cell suspension cultures of the model species Medicago truncatula A17
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|c 2023
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|a Text
|b txt
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|a ƒaComputermedien
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|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Revised 20.09.2023
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a © The Author(s) 2023.
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|a This manuscript describes a unique protocol for the rapid transformation of Medicago truncatula A17 cell suspension cultures mediated by Agrobacterium tumefaciens. Medicago cells were collected on day 7 of the growth curve, which corresponded to the beginning of the exponential phase. They were then co-cultured with Agrobacterium for 3 days before being spread onto a petri dish with appropriate antibiotic selection. The Receptor Binding Domain of the Spike protein of SARS-CoV-2 was used as a model to develop this protocol. The presence of the transgene was assessed using PCR, and the integrity of the product was evaluated by SDS-PAGE and Western-blotting
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|a Journal Article
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|a Agrobacterium
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|a Cultured cells
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|a Medicago
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|a Model legume
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|a Plant-based system
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|a RBD
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|a SARS-CoV-2
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| 650 |
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|a Stable transformation
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|a Rebelo, Bárbara A
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Abranches, Rita
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Plant cell, tissue and organ culture
|d 1987
|g 153(2023), 3 vom: 17., Seite 669-675
|w (DE-627)NLM098150995
|x 0167-6857
|7 nnas
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| 773 |
1 |
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|g volume:153
|g year:2023
|g number:3
|g day:17
|g pages:669-675
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|u http://dx.doi.org/10.1007/s11240-023-02495-6
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