NICOTIANAMINE SYNTHASE activity affects nucleolar iron accumulation and impacts rDNA silencing and RNA methylation in Arabidopsis

NICOTIANAMINE SYNTHASE activity affects nucleolar iron accumulation and impacts rDNA silencing and RNA methylation in Arabidopsis

© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.

Bibliographische Detailangaben
Veröffentlicht in:Journal of experimental botany. - 1985. - 74(2023), 15 vom: 17. Aug., Seite 4384-4400
1. Verfasser: Montacié, Charlotte (VerfasserIn)
Weitere Verfasser: Riondet, Christophe, Wei, Lili, Darrière, Tommy, Weiss, Alizée, Pontvianne, Frédéric, Escande, Marie-Line, de Bures, Anne, Jobet, Edouard, Barbarossa, Adrien, Carpentier, Marie-Christine, Aarts, Mark G M, Attina, Aurore, Hirtz, Christophe, David, Alexandre, Marchand, Virginie, Motorin, Yuri, Curie, Catherine, Mari, Stéphane, Reichheld, Jean-Philippe, Sáez-Vásquez, Julio
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Journal of experimental botany
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Iron methylation nicotianamine nucleolus rDNA redox nicotianamine synthase EC 2.5.- mehr... DNA, Ribosomal E1UOL152H7 RNA, Ribosomal
Beschreibung
Zusammenfassung:© The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology.
In plant cells, a large pool of iron (Fe) is contained in the nucleolus, as well as in chloroplasts and mitochondria. A central determinant for intracellular distribution of Fe is nicotianamine (NA) generated by NICOTIANAMINE SYNTHASE (NAS). Here, we used Arabidopsis thaliana plants with disrupted NAS genes to study the accumulation of nucleolar iron and understand its role in nucleolar functions and more specifically in rRNA gene expression. We found that nas124 triple mutant plants, which contained lower quantities of the iron ligand NA, also contained less iron in the nucleolus. This was concurrent with the expression of normally silenced rRNA genes from nucleolar organizer regions 2 (NOR2). Notably, in nas234 triple mutant plants, which also contained lower quantities of NA, nucleolar iron and rDNA expression were not affected. In contrast, in both nas124 and nas234, specific RNA modifications were differentially regulated in a genotype dependent manner. Taken together, our results highlight the impact of specific NAS activities in RNA gene expression. We discuss the interplay between NA and nucleolar iron with rDNA functional organization and RNA methylation
Beschreibung:Date Completed 18.08.2023
Date Revised 19.08.2023
published: Print
Citation Status MEDLINE
ISSN:1460-2431
DOI:10.1093/jxb/erad180