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231226s2023 xx |||||o 00| ||eng c |
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|a 10.2144/btn-2022-0022
|2 doi
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|a pubmed25n1188.xml
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|a (DE-627)NLM356641945
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|a DE-627
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|e rakwb
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|a eng
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1 |
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|a Hofmeister, Erik
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Comparison of magnetic bead and rapid swab RNA extraction methods for detecting rabbit hemorrhagic disease virus 2 in rabbit liver samples
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 19.05.2023
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|a Date Revised 14.06.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a We compared a bead RNA extraction method with a one-tube method that required only a heat block and ice. RNA was first extracted from liver samples from nine rabbits dying from rabbit hemorrhagic disease virus 2 (RHDV2) using magnetic beads, and RT-PCR was used to detect RHDV2 sequence. Following freezing, RNA was extracted a second time using the SwiftX™ Swabs Viral RNA Extraction Reagent. RHDV2 was detected in all nine samples. Cycle threshold values were higher in the RT-PCR following SwiftX extraction (mean: 3.79), indicating that the second extraction method resulted in approximately a 1 log10 reduction in sensitivity. A second freeze-thaw for the samples and less tissue extracted using SwiftX may have contributed additionally to the loss in sensitivity
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|a Letter
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|a RHDV2
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|a RNA extraction
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|a RNA virus detection
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4 |
|a field studies
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|a rabbit hemorrhagic disease virus 2
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4 |
|a rapid nucleic extraction methods
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| 650 |
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|a RNA, Viral
|2 NLM
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| 700 |
1 |
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|a Griffin, Kathryn
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Ip, Hon
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t BioTechniques
|d 1993
|g 74(2023), 4 vom: 30. Apr., Seite 156-157
|w (DE-627)NLM012627046
|x 1940-9818
|7 nnas
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| 773 |
1 |
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|g volume:74
|g year:2023
|g number:4
|g day:30
|g month:04
|g pages:156-157
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|u http://dx.doi.org/10.2144/btn-2022-0022
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