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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1002/aah.10187
|2 doi
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|a pubmed25n1187.xml
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|a (DE-627)NLM356430812
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|a (NLM)37139802
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Raji Sathyan, Krishnapriya
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Characterization of two tripartite motif-containing genes from Asian Seabass Lates calcarifer and their expression in response to virus infection and microbial molecular motifs
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| 264 |
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1 |
|c 2023
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| 336 |
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 23.10.2023
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|a Date Revised 30.10.2023
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|a published: Print-Electronic
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|a RefSeq: MN516801.1
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|a Citation Status MEDLINE
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|a © 2023 American Fisheries Society.
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|a OBJECTIVE: We identified two tripartite motif (TRIM) genes, LcTRIM21 and LcTRIM39, from the Asian Seabass Lates calcarifer, and examined their responses to experimental betanodavirus infection and stimulation with microbial pathogen-associated molecular patterns
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| 520 |
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|a METHODS: Genes encoding LcTRIM21 and LcTRIM39 were identified, cloned, and sequenced from the Asian Seabass. We analyzed the sequence using a variety of bioinformatics tools to determine protein structure, localization, and establish a phylogenetic tree. By using quantitative real-time PCR, we analyzed expression profiles of the LcTRIM21 and LcTRIM39 genes in response to betanodavirus challenge as well as molecular pathogen-associated molecular patterns like poly(I:C) and Zymosan A. The tissue distribution pattern of these genes was also examined in healthy animals
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|a RESULT: Asian Seabass homologues of the TRIM gene, LcTRIM21 and LcTRIM39, were cloned, both encoding proteins with 547 amino acids. LcTRIM21 is predicted to have an isoelectric point of 6.32 and a molecular mass of 62.11 kilodaltons, while LcTRIM39 has an isoelectric point of 5.57 and a molecular mass of 62.11 kilodaltons. LcTRIM21 and LcTRIM39 homologues were predicted to be localized in cytoplasm by in silico protein localization. Structurally, both proteins contain an N-terminal really interesting new gene (RING) zinc-finger domain, B-box domain, coiled-coil domain and C-terminal PRY/SPRY domain. Most tissues and organs examined showed constitutive expression of LcTRIM21 and LcTRIM39. Upon poly(I:C) challenge or red-spotted grouper nervous necrosis virus infection, LcTRIM21 and LcTRIM39 mRNA expression was significantly upregulated, suggesting that they may play a critical antiviral role against fish viruses. LcTRIM21 and LcTRIM39 expression were also upregulated by administration of the glucan Zymosan A
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|a CONCLUSION: The TRIM-containing gene is an E3 ubiquitin ligase that exhibits antiviral activity by targeting viral proteins via proteasome-mediated ubiquitination. TRIM proteins can be explored for the discovery of antivirals and strategies to combat diseases like viral nervous necrosis, that threaten seabass aquaculture
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a Lates calcarifer
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| 650 |
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4 |
|a TRIM21
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| 650 |
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|a TRIM39
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| 650 |
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4 |
|a RGNNV
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| 650 |
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4 |
|a Zymosan A
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| 650 |
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4 |
|a poly(I:C)
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| 650 |
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7 |
|a Pathogen-Associated Molecular Pattern Molecules
|2 NLM
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| 650 |
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7 |
|a Zymosan
|2 NLM
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| 650 |
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7 |
|a 9010-72-4
|2 NLM
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| 650 |
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7 |
|a Fish Proteins
|2 NLM
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| 650 |
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7 |
|a Poly I-C
|2 NLM
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| 650 |
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7 |
|a O84C90HH2L
|2 NLM
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| 650 |
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7 |
|a Antiviral Agents
|2 NLM
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| 700 |
1 |
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|a Premraj, Avinash
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thavarool Puthiyedathu, Sajeevan
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of aquatic animal health
|d 1998
|g 35(2023), 3 vom: 01. Sept., Seite 169-186
|w (DE-627)NLM098202006
|x 1548-8667
|7 nnas
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| 773 |
1 |
8 |
|g volume:35
|g year:2023
|g number:3
|g day:01
|g month:09
|g pages:169-186
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| 856 |
4 |
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|u http://dx.doi.org/10.1002/aah.10187
|3 Volltext
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|a AR
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|d 35
|j 2023
|e 3
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|c 09
|h 169-186
|