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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.107706
|2 doi
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|a pubmed24n1187.xml
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|a (DE-627)NLM356229386
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|a (NLM)37119548
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|a (PII)S0981-9428(23)00217-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Shen, Qing-Qing
|e verfasserin
|4 aut
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|a The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 23.05.2023
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|a Date Revised 23.05.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier Masson SAS. All rights reserved.
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|a In this study, we characterized a WRKY family member gene, SsWRKY1, which is located in the nucleus and contains multiple stress-related cis-acting elements. In addition, constructed SsWRKY1-overexpressing Arabidopsis thaliana had higher antioxidant enzyme activity and proline content under drought stress conditions, with lower malondialdehyde content and reactive oxygen species (ROS) accumulation, and the expression levels of six stress-related genes were significantly upregulated. This indicates that the overexpression of SsWRKY1 in Arabidopsis thaliana improves resistance to drought stress. SsWRKY1 does not have transcriptional autoactivation activity in yeast cells. The yeast two-hybrid (Y2H) system and the S. spontaneum cDNA library were used to screen 21 potential proteins that interact with SsWRKY1, and the interaction between SsWRKY1 and ATAF2 was verified by GST pull-down assay. In summary, our results indicate that SsWRKY1 plays an important role in the response to drought stress and provide initial insights into the molecular mechanism of SsWRKY1 in response to drought stress
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|a Journal Article
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|a Drought tolerance
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|a Protein interaction
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|a Sugarcane
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|a WRKY transcription Factors
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|a Transcription Factors
|2 NLM
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|a Arabidopsis Proteins
|2 NLM
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|a Plant Proteins
|2 NLM
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|a Antioxidants
|2 NLM
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|a Wang, Tian-Ju
|e verfasserin
|4 aut
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|a Wang, Jun-Gang
|e verfasserin
|4 aut
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|a He, Li-Lian
|e verfasserin
|4 aut
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|a Zhao, Ting-Ting
|e verfasserin
|4 aut
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|a Zhao, Xue-Ting
|e verfasserin
|4 aut
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|a Xie, Lin-Yan
|e verfasserin
|4 aut
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|a Qian, Zhen-Feng
|e verfasserin
|4 aut
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|a Wang, Xian-Hong
|e verfasserin
|4 aut
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|a Liu, Lu-Feng
|e verfasserin
|4 aut
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|a Chen, Shu-Ying
|e verfasserin
|4 aut
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|a Zhang, Shu-Zhen
|e verfasserin
|4 aut
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|a Li, Fu-Sheng
|e verfasserin
|4 aut
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 199(2023) vom: 13. Juni, Seite 107706
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnns
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|g volume:199
|g year:2023
|g day:13
|g month:06
|g pages:107706
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|u http://dx.doi.org/10.1016/j.plaphy.2023.107706
|3 Volltext
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