The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum

Copyright © 2023 Elsevier Masson SAS. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Plant physiology and biochemistry : PPB. - 1991. - 199(2023) vom: 13. Juni, Seite 107706
1. Verfasser: Shen, Qing-Qing (VerfasserIn)
Weitere Verfasser: Wang, Tian-Ju, Wang, Jun-Gang, He, Li-Lian, Zhao, Ting-Ting, Zhao, Xue-Ting, Xie, Lin-Yan, Qian, Zhen-Feng, Wang, Xian-Hong, Liu, Lu-Feng, Chen, Shu-Ying, Zhang, Shu-Zhen, Li, Fu-Sheng
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Plant physiology and biochemistry : PPB
Schlagworte:Journal Article Drought tolerance Protein interaction Sugarcane WRKY transcription Factors Transcription Factors Arabidopsis Proteins Plant Proteins Antioxidants
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245 1 4 |a The SsWRKY1 transcription factor of Saccharum spontaneum enhances drought tolerance in transgenic Arabidopsis thaliana and interacts with 21 potential proteins to regulate drought tolerance in S. spontaneum 
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520 |a In this study, we characterized a WRKY family member gene, SsWRKY1, which is located in the nucleus and contains multiple stress-related cis-acting elements. In addition, constructed SsWRKY1-overexpressing Arabidopsis thaliana had higher antioxidant enzyme activity and proline content under drought stress conditions, with lower malondialdehyde content and reactive oxygen species (ROS) accumulation, and the expression levels of six stress-related genes were significantly upregulated. This indicates that the overexpression of SsWRKY1 in Arabidopsis thaliana improves resistance to drought stress. SsWRKY1 does not have transcriptional autoactivation activity in yeast cells. The yeast two-hybrid (Y2H) system and the S. spontaneum cDNA library were used to screen 21 potential proteins that interact with SsWRKY1, and the interaction between SsWRKY1 and ATAF2 was verified by GST pull-down assay. In summary, our results indicate that SsWRKY1 plays an important role in the response to drought stress and provide initial insights into the molecular mechanism of SsWRKY1 in response to drought stress 
650 4 |a Journal Article 
650 4 |a Drought tolerance 
650 4 |a Protein interaction 
650 4 |a Sugarcane 
650 4 |a WRKY transcription Factors 
650 7 |a Transcription Factors  |2 NLM 
650 7 |a Arabidopsis Proteins  |2 NLM 
650 7 |a Plant Proteins  |2 NLM 
650 7 |a Antioxidants  |2 NLM 
700 1 |a Wang, Tian-Ju  |e verfasserin  |4 aut 
700 1 |a Wang, Jun-Gang  |e verfasserin  |4 aut 
700 1 |a He, Li-Lian  |e verfasserin  |4 aut 
700 1 |a Zhao, Ting-Ting  |e verfasserin  |4 aut 
700 1 |a Zhao, Xue-Ting  |e verfasserin  |4 aut 
700 1 |a Xie, Lin-Yan  |e verfasserin  |4 aut 
700 1 |a Qian, Zhen-Feng  |e verfasserin  |4 aut 
700 1 |a Wang, Xian-Hong  |e verfasserin  |4 aut 
700 1 |a Liu, Lu-Feng  |e verfasserin  |4 aut 
700 1 |a Chen, Shu-Ying  |e verfasserin  |4 aut 
700 1 |a Zhang, Shu-Zhen  |e verfasserin  |4 aut 
700 1 |a Li, Fu-Sheng  |e verfasserin  |4 aut 
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773 1 8 |g volume:199  |g year:2023  |g day:13  |g month:06  |g pages:107706 
856 4 0 |u http://dx.doi.org/10.1016/j.plaphy.2023.107706  |3 Volltext 
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