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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.3c00469
|2 doi
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|a pubmed24n1187.xml
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|a (DE-627)NLM356198707
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|a (NLM)37116313
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|a eng
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|a Li, Na
|e verfasserin
|4 aut
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|a Exploring the pH Sensitivity of Ion-Pair Interactions on a Self-Assembled Monolayer by Scanning Electrochemical Microscopy
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|c 2023
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|a Text
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|a ƒaComputermedien
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|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 09.05.2023
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|a Date Revised 09.05.2023
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a Insights into the chemical essence of weak interactions on the surface of biomacromolecules may help to regulate biological processes. In this work, the pH sensitivity of ion-pair interactions occurring on a cysteine self-assembled monolayer (Cys SAM) that simulates the local surface of a protein was probed by scanning electrochemical microscopy (SECM). Cys SAM and the ion-pair interactions subsequently formed with the introduced aspartic acid (Asp) were both pH-sensitive, as confirmed by the tip current changes in the feedback mode. After continuous pH measurements, the most significant negative feedback was observed at pH 5.50, indicating the most robust ion-pair interactions, which were simultaneously identified by voltammetry. In this case, the extra addition of the inorganic cation (i.e., Ca2+) did not disrupt the existing ion-pair interactions, and the binding constant (K) and Gibbs free energy (ΔGo) of the ion pair were finally determined to be 6.44 × 105 M-1 and -33.14 kJ mol-1, respectively. Overall, the pH sensitivity of ion-pair interactions was found to be mainly attributable to pH-induced changes in the deprotonated/protonated states of the α-amino acid moieties, which may provide insights into the artificial manipulation of complex binding events at the molecular level on the biological surface
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|a Journal Article
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|a Huang, Ximing
|e verfasserin
|4 aut
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|a Shao, Huibo
|e verfasserin
|4 aut
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 39(2023), 18 vom: 09. Mai, Seite 6529-6538
|w (DE-627)NLM098181009
|x 1520-5827
|7 nnns
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| 773 |
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|g volume:39
|g year:2023
|g number:18
|g day:09
|g month:05
|g pages:6529-6538
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|u http://dx.doi.org/10.1021/acs.langmuir.3c00469
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