Effect of Vicia sativa L. on Motility, Mortality and Expression Levels of hsp Genes in J2 Stage of Meloidogyne hapla

© 2023 Dobosz et al., published by Sciendo.

Bibliographische Detailangaben
Veröffentlicht in:Journal of nematology. - 1969. - 55(2023), 1 vom: 01. Feb., Seite 20230009
1. Verfasser: Dobosz, Renata (VerfasserIn)
Weitere Verfasser: Flis, Łukasz, Bocianowski, Jan, Malewski, Tadeusz
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Journal of nematology
Schlagworte:Journal Article Meloidogyne hapla Vicia sativa seeds behavior hsp genes mortality motility
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520 |a Assuming that the seeds of Vicia sativa L. have a stressful effect on J2 stage Meloidogyne hapla, we undertook research on the effect of these seeds on the motility and mortality of J2 and determined the expression levels of selected hsp genes in J2. The assessment of the effect of V. sativa seeds on the motility of M. hapla specimens consisted of observing the movement of J2 immersed in a seed diffusate or in a tomato root filtrate at temperatures of 10, 17, and 21°C. In J2 treated with V. sativa (cv. Ina) seed diffusates, the expression level of hsp genes was determined by qPCR. J2 exposed to V. sativa diffusates were found to lose their motility, while their mortality did not exceed 30%. J2 in the seed diffusate were characterized by an increase in the expression levels of the Mh-hsp90, Mh-hsp1, and Mh-hsp43 genes. It is suggested that the hsp90 gene may be a potential bioindicator of the environmental impact on Meloidogyne nematodes. The impaired ability to move in J2 of M. hapla is attributable to the occurrence of V. sativa seeds in their habitat. These studies may contribute to developing methods of reducing crop damage caused by M. hapla 
650 4 |a Journal Article 
650 4 |a Meloidogyne hapla 
650 4 |a Vicia sativa seeds 
650 4 |a behavior 
650 4 |a hsp genes 
650 4 |a mortality 
650 4 |a motility 
700 1 |a Flis, Łukasz  |e verfasserin  |4 aut 
700 1 |a Bocianowski, Jan  |e verfasserin  |4 aut 
700 1 |a Malewski, Tadeusz  |e verfasserin  |4 aut 
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