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20231226065155.0 |
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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1094/PDIS-11-22-2570-SC
|2 doi
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|a pubmed24n1186.xml
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|a (DE-627)NLM355828065
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|a (NLM)37079007
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Zhao, Yue
|e verfasserin
|4 aut
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| 245 |
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|a Pitaya Virus X Coat Protein Acts as an RNA Silencing Suppressor and Can Be Used as a Specific Target for Detection Using RT-LAMP
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 23.11.2023
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|a Date Revised 23.11.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Selenicereus undatus (Haworth) D.R. Hunt (pitaya) is a tropical fruit that has been commonly cultivated in Guizhou Province, China, in recent years due to its good taste and high nutritional value. This planting area currently ranks third in China. Viral diseases have increasingly emerged in pitaya cultivation because of the expansion of the pitaya planting area and the characteristics of asexual propagation. The spread of pitaya virus X (PiVX; a Potexvirus) is among the most severe viruses threatening the quality and yield of pitaya fruit. To investigate the occurrence of PiVX in pitaya cultivations in Guizhou Province, we developed a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method that can detect PiVX with high sensitivity and specificity at a low cost and produce a visualized result. Our best RT-LAMP system was significantly more sensitive than RT-PCR and was highly specific to PiVX. Furthermore, PiVX coat protein (CP) can form a homodimer, and PiVX may use its CP as a plant RNA silencing suppressor to enhance infection. To the best of our knowledge, this is the first report of fast detection of PiVX and functional exploration of CP in a Potexvirus. These findings will provide an opportunity for early diagnosis and prevention of viruses in pitaya
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|a Journal Article
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|a RNA silencing suppressor
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|a RT-LAMP
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|a coat protein
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|a pitaya
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|a pitaya virus X
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| 650 |
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4 |
|a protein-protein interaction
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| 700 |
1 |
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|a Yang, Shutong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Ling
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yang, Qian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Luo, Liting
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Jiang, Junmei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Malichan, Srihunsa
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhao, Jin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xie, Xin
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant disease
|d 1997
|g 107(2023), 11 vom: 20. Nov., Seite 3378-3382
|w (DE-627)NLM098181742
|x 0191-2917
|7 nnns
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| 773 |
1 |
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|g volume:107
|g year:2023
|g number:11
|g day:20
|g month:11
|g pages:3378-3382
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|u http://dx.doi.org/10.1094/PDIS-11-22-2570-SC
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