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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.jplph.2023.153981
|2 doi
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|a pubmed25n1184.xml
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|a (DE-627)NLM355589214
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|a (NLM)37054580
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|a (PII)S0176-1617(23)00075-5
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Lee, Joo Sun
|e verfasserin
|4 aut
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| 245 |
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|a Oat AsDA1-2D enhances heat stress tolerance and negatively regulates seed-storage globulin
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
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|2 rdacarrier
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|a Date Completed 26.04.2023
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|a Date Revised 26.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Copyright © 2023 Elsevier GmbH. All rights reserved.
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|a The importance of oats has increased because of their high nutritional value and health benefits in the human diet. High-temperature stress during the reproductive growth period has a detrimental effect on grain morphology by changing the structure and concentration of several seed-storage proteins. DA1, a conserved ubiquitin-proteasome pathway component, plays an important role in regulating grain size by controlling cell proliferation in maternal integuments during the grain-filling stage. However, there have been no reports or studies on oat DA1 genes. In this study, we identified three DA1-like genes (AsDA1-2D, AsDA1-5A, and AsDA1-1D) using genome-wide analysis. Among these, AsDA1-2D was found to be responsible for high-temperature stress tolerance via a yeast thermotolerance assay. The physical interaction of AsDA1-2D with oat-storage-globulin (AsGL-4D) and a protease inhibitor (AsPI-4D) was observed using yeast two-hybrid screening. A subcellular localization assay revealed that AsDA1-2D and its interacting proteins are localized in the cytosol and plasma membrane. An in vitro pull-down assay showed that AsDA1-2D forms a complex with both AsPI-4D and AsGL-4D. An in vitro cell-free degradation assay showed that AsGL-4D was degraded by AsDA1-2D under high-temperature conditions and that AsPI-4D inhibited the function of AsDA1-2D. These results suggest that AsDA1-2D acts as a cysteine protease and negatively regulates oat-grain-storage-globulin under heat stress
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|a Journal Article
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|a DA1
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|a Globulin
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|a Grain-filling period
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| 650 |
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|a High-temperature stress
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|a Oat
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|a Protease inhibitor
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| 650 |
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|a Globulins
|2 NLM
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| 700 |
1 |
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|a Ko, Chan Seop
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Seo, Yong Weon
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Journal of plant physiology
|d 1979
|g 284(2023) vom: 05. Mai, Seite 153981
|w (DE-627)NLM098174622
|x 1618-1328
|7 nnas
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| 773 |
1 |
8 |
|g volume:284
|g year:2023
|g day:05
|g month:05
|g pages:153981
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| 856 |
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|u http://dx.doi.org/10.1016/j.jplph.2023.153981
|3 Volltext
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