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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1021/acs.langmuir.2c03306
|2 doi
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|a eng
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| 100 |
1 |
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|a Crothers, Ruth A
|e verfasserin
|4 aut
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| 245 |
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|a Characterization and Optimization of Fluorescent Organosilica Colloids for 3D Confocal Microscopy Prepared Under "Zero-Flow" Conditions
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|c 2023
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|a Text
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 18.04.2023
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|a Date Revised 18.04.2023
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a We optimize and characterize the preparation of 3-trimethoxysilyl propyl methacrylate (TPM) colloidal suspensions for three-dimensional confocal microscopy. We revisit a simple synthesis of TPM microspheres by nucleation of droplets from prehydrolyzed TPM oil in a "zero-flow" regime and demonstrate how precise and reproducible control of particle size may be achieved via single-step nucleation with a focus on how the reagents are mixed. We also revamp the conventional dyeing method for TPM particles to achieve uniform transfer of a fluorophore to the organosilica droplets, improving particle identification. Finally, we illustrate how a ternary mixture of tetralin, trichloroethylene, and tetrachloroethylene may be used as a suspension medium which matches the refractive index of these particles while allowing independent control of the density mismatch between particle and solvent
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|a Journal Article
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|a Orr, Nicholas H P
|e verfasserin
|4 aut
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|a van der Meer, Berend
|e verfasserin
|4 aut
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|a Dullens, Roel P A
|e verfasserin
|4 aut
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|a Yanagishima, Taiki
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Langmuir : the ACS journal of surfaces and colloids
|d 1992
|g 39(2023), 15 vom: 18. Apr., Seite 5306-5314
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|g year:2023
|g number:15
|g day:18
|g month:04
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|u http://dx.doi.org/10.1021/acs.langmuir.2c03306
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