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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/nph.18910
|2 doi
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|a pubmed24n1182.xml
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|a (DE-627)NLM354726420
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|a (NLM)36967582
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Nieves-Cordones, Manuel
|e verfasserin
|4 aut
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| 245 |
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|a Inhibition of SlSKOR by SlCIPK23-SlCBL1/9 uncovers CIPK-CBL-target network rewiring in land plants
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 19.05.2023
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|a Date Revised 21.05.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 The Authors New Phytologist © 2023 New Phytologist Foundation.
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|a Transport of K+ to the xylem is a key process in the mineral nutrition of the shoots. Although CIPK-CBL complexes have been widely shown to regulate K+ uptake transport systems, no information is available about the xylem ones. Here, we studied the physiological roles of the voltage-gated K+ channel SlSKOR and its regulation by the SlCIPK23-SlCBL1/9 complexes in tomato plants. We phenotyped gene-edited slskor and slcipk23 tomato knockout mutants and carried out two-electrode voltage-clamp (TEVC) and BiFC assays in Xenopus oocytes as key approaches. SlSKOR was preferentially expressed in the root stele and was important not only for K+ transport to shoots but also, indirectly, for that of Ca2+ , Mg2+ , Na+ , NO3 - , and Cl- . Surprisingly, the SlCIPK23-SlCBL1/9 complexes turned out to be negative regulators of SlSKOR. Inhibition of SlSKOR by SlCIPK23-SlCBL1/9 was observed in Xenopus oocytes and tomato plants. Regulation of SKOR-like channels by CIPK23-CBL1 complexes was also present in Medicago, grapevine, and lettuce but not in Arabidopsis and saltwater cress. Our results provide a molecular framework for coordinating root K+ uptake and its translocation to the shoot by SlCIPK23-SlCBL1/9 in tomato plants. Moreover, they evidenced that CIPK-CBL-target networks have evolved differently in land plants
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a CIPK-CBL
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|a CRISPR-Cas
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|a potassium
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|a salinity
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|a tomato
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|a voltage-gated channel
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|a xylem
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|a Arabidopsis Proteins
|2 NLM
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| 650 |
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|a Potassium Channels
|2 NLM
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| 650 |
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|a Potassium
|2 NLM
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| 650 |
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|a RWP5GA015D
|2 NLM
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| 650 |
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|a Plant Proteins
|2 NLM
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| 700 |
1 |
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|a Amo, Jesús
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hurtado-Navarro, Laura
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez-Martínez, Almudena
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Martínez, Vicente
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Rubio, Francisco
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t The New phytologist
|d 1979
|g 238(2023), 6 vom: 05. Juni, Seite 2495-2511
|w (DE-627)NLM09818248X
|x 1469-8137
|7 nnns
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| 773 |
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|g volume:238
|g year:2023
|g number:6
|g day:05
|g month:06
|g pages:2495-2511
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|u http://dx.doi.org/10.1111/nph.18910
|3 Volltext
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|d 238
|j 2023
|e 6
|b 05
|c 06
|h 2495-2511
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