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20231226062818.0 |
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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1111/jfd.13785
|2 doi
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|a pubmed24n1182.xml
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|a (DE-627)NLM354714562
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|a (NLM)36966380
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|a DE-627
|b ger
|c DE-627
|e rakwb
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| 041 |
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|a eng
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| 100 |
1 |
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|a Liao, Jiaming
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Establishment and characterization of a brain tissue cell line from spotted knifejaw (Oplegnathus punctatus) and its susceptibility to several fish viruses
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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| 338 |
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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| 500 |
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|a Date Completed 07.06.2023
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|a Date Revised 07.06.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a © 2023 John Wiley & Sons Ltd.
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|a Cells are important in the study of virus isolation and identification, viral pathogenic mechanisms and antiviral immunity. The spotted knifejaw (Oplegnathus punctatus) is a significant farmed fish in China that has been greatly affected by diseases in recent years. In this study, a new cell line derived from the spotted knifejaw brain (SKB) was established and characterized. SKB cells multiplied well in Leibovitz's L-15 medium supplemented with 10% fetal bovine serum at 28°C. Chromosome analysis revealed that modal chromosome number was 48 for SKB. SKB cells exhibit susceptibility to several fish viruses, such as a largemouth bass virus, red grouper nervous necrosis virus (RGNNV), infectious spleen and kidney necrosis virus (ISKNV), Singapore grouper iridovirus (SGIV) and spotted knifejaw iridovirus isolate (SKIV-TJ), as shown by cytopathic effect and increased viral titers. Electron microscopy results showed that the cytoplasm contained a large number of vacuoles, and many virus particles existed at the edge of the vacuoles in RGNNV-infected cells and numerous viral particles were scattered throughout the cytoplasm in both ISKNV- and SKIV-TJ-infected cells. These results suggest that SKB is an ideal tool for studying host-virus interactions and potential vaccine development
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4 |
|a Journal Article
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| 650 |
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|a Oplegnathus punctatus
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| 650 |
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|a chromosome analysis
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| 650 |
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|a immune-related genes
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| 650 |
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4 |
|a spotted knifejaw brain
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| 650 |
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4 |
|a virus susceptibility
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| 650 |
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7 |
|a Fish Proteins
|2 NLM
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| 700 |
1 |
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|a Zhang, Xin
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Kang, Shaozhu
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Luhao
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Zhang, Dongzhuo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xu, Zhuqing
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Qin, Qiwei
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wei, Jingguang
|e verfasserin
|4 aut
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| 773 |
0 |
8 |
|i Enthalten in
|t Journal of fish diseases
|d 1998
|g 46(2023), 7 vom: 26. Juli, Seite 767-777
|w (DE-627)NLM098166034
|x 1365-2761
|7 nnns
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| 773 |
1 |
8 |
|g volume:46
|g year:2023
|g number:7
|g day:26
|g month:07
|g pages:767-777
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| 856 |
4 |
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|u http://dx.doi.org/10.1111/jfd.13785
|3 Volltext
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|d 46
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|h 767-777
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