Modular Synthesis of Semiconducting Graft Copolymers to Achieve "Clickable" Fluorescent Nanoparticles with Long Circulation and Specific Cancer Targeting

Modular Synthesis of Semiconducting Graft Copolymers to Achieve "Clickable" Fluorescent Nanoparticles with Long Circulation and Specific Cancer Targeting

© 2023 The Authors. Advanced Materials published by Wiley‐VCH GmbH.

Bibliographische Detailangaben
Veröffentlicht in:Advanced materials (Deerfield Beach, Fla.). - 1998. - 36(2024), 20 vom: 08. Mai, Seite e2300413
1. Verfasser: Creamer, Adam (VerfasserIn)
Weitere Verfasser: Fiego, Alessandra Lo, Agliano, Alice, Prados-Martin, Lino, Høgset, Håkon, Najer, Adrian, Richards, Daniel A, Wojciechowski, Jonathan P, Foote, James E J, Kim, Nayoung, Monahan, Amy, Tang, Jiaqing, Shamsabadi, André, Rochet, Léa N C, Thanasi, Ioanna A, de la Ballina, Laura R, Rapley, Charlotte L, Turnock, Stephen, Love, Elizabeth A, Bugeon, Laurence, Dallman, Margaret J, Heeney, Martin, Kramer-Marek, Gabriela, Chudasama, Vijay, Fenaroli, Federico, Stevens, Molly M
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2024
Zugriff auf das übergeordnete Werk:Advanced materials (Deerfield Beach, Fla.)
Schlagworte:Journal Article fluorescent nanoparticles graft copolymers polymer brushes polymer dots semiconducting polymer nanoparticles
Beschreibung
Zusammenfassung:© 2023 The Authors. Advanced Materials published by Wiley‐VCH GmbH.
Semiconducting polymer nanoparticles (SPNs) are explored for applications in cancer theranostics because of their high absorption coefficients, photostability, and biocompatibility. However, SPNs are susceptible to aggregation and protein fouling in physiological conditions, which can be detrimental for in vivo applications. Here, a method for achieving colloidally stable and low-fouling SPNs is described by grafting poly(ethylene glycol) (PEG) onto the backbone of the fluorescent semiconducting polymer, poly(9,9'-dioctylfluorene-5-fluoro-2,1,3-benzothiadiazole), in a simple one-step substitution reaction, postpolymerization. Further, by utilizing azide-functionalized PEG, anti-human epidermal growth factor receptor 2 (HER2) antibodies, antibody fragments, or affibodies are site-specifically "clicked" onto the SPN surface, which allows the functionalized SPNs to specifically target HER2-positive cancer cells. In vivo, the PEGylated SPNs are found to have excellent circulation efficiencies in zebrafish embryos for up to seven days postinjection. SPNs functionalized with affibodies are then shown to be able to target HER2 expressing cancer cells in a zebrafish xenograft model. The covalent PEGylated SPN system described herein shows great potential for cancer theranostics
Beschreibung:Date Completed 16.05.2024
Date Revised 13.12.2024
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-4095
DOI:10.1002/adma.202300413