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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1107/S1600577523000462
|2 doi
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|a pubmed24n1533.xml
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|a (DE-627)NLM353975737
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|a (NLM)36891848
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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|a Zhao, Feng Zhu
|e verfasserin
|4 aut
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|a Microfluidic rotating-target device capable of three-degrees-of-freedom motion for efficient in situ serial synchrotron crystallography
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 09.03.2023
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|a Date Revised 14.09.2024
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|a published: Print-Electronic
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|a Citation Status PubMed-not-MEDLINE
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|a open access.
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|a There is an increasing demand for simple and efficient sample delivery technology to match the rapid development of serial crystallography and its wide application in analyzing the structural dynamics of biological macromolecules. Here, a microfluidic rotating-target device is presented, capable of three-degrees-of-freedom motion, including two rotational degrees of freedom and one translational degree of freedom, for sample delivery. Lysozyme crystals were used as a test model with this device to collect serial synchrotron crystallography data and the device was found to be convenient and useful. This device enables in situ diffraction from crystals in a microfluidic channel without the need for crystal harvesting. The circular motion ensures that the delivery speed can be adjusted over a wide range, showing its good compatibility with different light sources. Moreover, the three-degrees-of-freedom motion guarantees the full utilization of crystals. Hence, sample consumption is greatly reduced, and only 0.1 mg of protein is consumed in collecting a complete dataset
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|a Journal Article
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|a circular motion
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|a in situ diffraction
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|a microfluidic rotating-target
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|a sample delivery system
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|a serial synchrotron crystallography
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|a Wang, Zhi Jun
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Xiao, Qing Jie
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yu, Li
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Sun, Bo
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Hou, Qian
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Chen, Liang Liang
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Liang, Huan
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wu, Hai
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Guo, Wei Hong
|e verfasserin
|4 aut
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| 700 |
1 |
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|a He, Jian Hua
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Wang, Qi Sheng
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Yin, Da Chuan
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of synchrotron radiation
|d 1994
|g 30(2023), Pt 2 vom: 01. März, Seite 347-358
|w (DE-627)NLM09824129X
|x 1600-5775
|7 nnns
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| 773 |
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|g volume:30
|g year:2023
|g number:Pt 2
|g day:01
|g month:03
|g pages:347-358
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|u http://dx.doi.org/10.1107/S1600577523000462
|3 Volltext
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|d 30
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|e Pt 2
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|c 03
|h 347-358
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