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231226s2023 xx |||||o 00| ||eng c |
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|a 10.1016/j.plaphy.2023.02.040
|2 doi
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|a pubmed25n1179.xml
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|a (DE-627)NLM353949574
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|a (NLM)36889233
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|a (PII)S0981-9428(23)00114-6
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|a DE-627
|b ger
|c DE-627
|e rakwb
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|a eng
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| 100 |
1 |
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|a Shockey, Jay
|e verfasserin
|4 aut
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| 245 |
1 |
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|a Assessing the biotechnological potential of cotton type-1 and type-2 diacylglycerol acyltransferases in transgenic systems
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
|b c
|2 rdamedia
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|a ƒa Online-Ressource
|b cr
|2 rdacarrier
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|a Date Completed 05.04.2023
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|a Date Revised 05.04.2023
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|a published: Print-Electronic
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|a Citation Status MEDLINE
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|a Published by Elsevier Masson SAS.
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|a The chemical and physical properties of vegetable oils are largely dictated by the ratios of 4-6 common fatty acids contained within each oil. However, examples of plant species that accumulate from trace amounts to >90% of certain unusual fatty acids in seed triacylglycerols have been reported. Many of the general enzymatic reactions that drive both common and unusual fatty acid biosynthesis and accumulation in stored lipids are known, but which isozymes have evolved to specifically fill this role and how they coordinate in vivo is still poorly understood. Cotton (Gossypium sp.) is the very rare example of a commodity oilseed that produces biologically relevant amounts of unusual fatty acids in its seeds and other organs. In this case, unusual cyclopropyl fatty acids (named after the cyclopropane and cyclopropene moieties within the fatty acids) are found in membrane and storage glycerolipids (e.g. seed oils). Such fatty acids are useful in the synthesis of lubricants, coatings, and other types of valuable industrial feedstocks. To characterize the role of cotton acyltransferases in cyclopropyl fatty acid accumulation for bioengineering applications, we cloned and characterized type-1 and type-2 diacylglycerol acyltransferases from cotton and compared their biochemical properties to that of litchi (Litchi chinensis), another cyclopropyl fatty acid-producing plant. The results presented from transgenic microbes and plants indicate both cotton DGAT1 and DGAT2 isozymes efficiently utilize cyclopropyl fatty acid-containing substrates, which helps to alleviate biosynthetic bottlenecks and enhances total cyclopropyl fatty acid accumulation in the seed oil
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|a Journal Article
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|a Acyl-CoA
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|a Cyclopropane synthase
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|a Cyclopropyl fatty acids
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|a Diacylglycerol acyltransferase
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|a Phosphatidylcholine
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|a Triacylglycerol
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|a Diacylglycerol O-Acyltransferase
|2 NLM
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|a EC 2.3.1.20
|2 NLM
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|a Diglycerides
|2 NLM
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|a Isoenzymes
|2 NLM
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| 650 |
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|a Acyltransferases
|2 NLM
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| 650 |
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|a EC 2.3.-
|2 NLM
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| 650 |
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|a Fatty Acids
|2 NLM
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| 650 |
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|a Triglycerides
|2 NLM
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| 650 |
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|a Plant Oils
|2 NLM
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| 700 |
1 |
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|a Parchuri, Prasad
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Thyssen, Gregory N
|e verfasserin
|4 aut
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| 700 |
1 |
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|a Bates, Philip D
|e verfasserin
|4 aut
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| 773 |
0 |
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|i Enthalten in
|t Plant physiology and biochemistry : PPB
|d 1991
|g 196(2023) vom: 01. März, Seite 940-951
|w (DE-627)NLM098178261
|x 1873-2690
|7 nnas
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| 773 |
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|g volume:196
|g year:2023
|g day:01
|g month:03
|g pages:940-951
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| 856 |
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|u http://dx.doi.org/10.1016/j.plaphy.2023.02.040
|3 Volltext
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|d 196
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