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|a 10.1093/jxb/erad072
|2 doi
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|a pubmed25n1178.xml
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|a DE-627
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|a eng
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|a Guzmán-Benito, Irene
|e verfasserin
|4 aut
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| 245 |
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|a CRISPR/Cas-mediated in planta gene targeting
|b current advances and challenges
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|c 2023
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|a Text
|b txt
|2 rdacontent
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|a ƒaComputermedien
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|a ƒa Online-Ressource
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|a Date Completed 21.07.2023
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|a Date Revised 24.07.2023
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|a published: Print
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|a Citation Status MEDLINE
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|a © The Author(s) 2023. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissionsoup.com.
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|a Gene targeting can be used to make modifications at a specific region in a plant's genome and create high-precision tools for plant biotechnology and breeding. However, its low efficiency is a major barrier to its use in plants. The discovery of CRISPR (clustered regularly interspaced short palindromic repeats)-Cas-based site-specific nucleases capable of inducing double-strand breaks in desired loci resulted in the development of novel approaches for plant gene targeting. Several studies have recently demonstrated improvements in gene targeting efficiency through cell-type-specific expression of Cas nucleases, the use of self-amplified gene-targeting-vector DNA, or manipulation of RNA silencing and DNA repair pathways. In this review, we summarize recent advances in CRISPR/Cas-mediated gene targeting in plants and discuss potential efficiency improvements. Increasing the efficiency of gene targeting technology will help pave the way for increased crop yields and food safety in environmentally friendly agriculture
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|a Review
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|a Journal Article
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|a Research Support, Non-U.S. Gov't
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|a CRISPR–Cas
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|a DNA sequence knock-in
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|a DNA sequence replacement
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|a homologous recombination
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|a plant gene targeting
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|a Endonucleases
|2 NLM
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|a EC 3.1.-
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|a Achkar, Natalia Patricia
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|4 aut
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|a Bologna, Nicolas G
|e verfasserin
|4 aut
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| 700 |
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|a Ursache, Robertas
|e verfasserin
|4 aut
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| 773 |
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|i Enthalten in
|t Journal of experimental botany
|d 1985
|g 74(2023), 13 vom: 18. Juli, Seite 3806-3820
|w (DE-627)NLM098182706
|x 1460-2431
|7 nnas
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|g volume:74
|g year:2023
|g number:13
|g day:18
|g month:07
|g pages:3806-3820
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|u http://dx.doi.org/10.1093/jxb/erad072
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