Inhibition of ERAP1 represses HLA-B27 free heavy chains expression on polarized macrophages and interrupts NK cells activation and function from ankylosing spondylitis

Inhibition of ERAP1 represses HLA-B27 free heavy chains expression on polarized macrophages and interrupts NK cells activation and function from ankylosing spondylitis

Copyright © 2023 Elsevier Inc. All rights reserved.

Bibliographische Detailangaben
Veröffentlicht in:Clinical immunology (Orlando, Fla.). - 1999. - 248(2023) vom: 20. März, Seite 109268
1. Verfasser: Babaie, Farhad (VerfasserIn)
Weitere Verfasser: Mohammadi, Hamed, Salimi, Sorayya, Ghanavatinegad, Alireza, Abbasifard, Mitra, Yousefi, Mehdi, Hajaliloo, Mehrzad, Khalili, Younes, Zamanlou, Sajjad, Safari, Roghaiyeh, Hemmatzadeh, Maryam, Rezaiemanesh, Alireza, Salimi, Reza, Baradaran, Behzad, Babaloo, Zohreh
Format: Online-Aufsatz
Sprache:English
Veröffentlicht: 2023
Zugriff auf das übergeordnete Werk:Clinical immunology (Orlando, Fla.)
Schlagworte:Journal Article Research Support, Non-U.S. Gov't Ankylosing spondylitis Endoplasmic reticulum aminopeptidase 1 Free heavy chains Human leukocyte antigen-B27 Macrophage Natural killer cell HLA-B27 Antigen Minor Histocompatibility Antigens mehr... ERAP1 protein, human EC 3.4.11.- Aminopeptidases
Beschreibung
Zusammenfassung:Copyright © 2023 Elsevier Inc. All rights reserved.
BACKGROUND: We aimed to assess if Endoplasmic reticulum aminopeptidase 1 (ERAP1) polymorphisms might impress Human leukocyte antigen (HLA)-B27-free heavy chains (FHCs) expression on macrophages and eventually NK cell activation in Ankylosing spondylitis (AS)
METHODS: Blood samples were obtained from 10 HLAB27+ patients with protective and 10 HLAB27+ patients with non-protective genotype. Monocytes were isolated and polarized toward M1 and M2 macrophages. ERAP1 was inhibited in macrophages, which were then co-cultured with autologous NK cells
RESULTS: Expression of HLA-B27-FHCs on M1 and M2 macrophages was reduced in patients with protective ERAP1 genotype. Co-culturing ERAP1-inhibited M1 macrophages and NK cells from patients with protective genotype resulted in downmodulation of CD69 and CD107a markers on NK cells and reduced number of IFN-γ+ NK cells compared to that of patients with non-protective genotypes
CONCLUSION: Inhibition of ERAP1 activity, by diminishing NK activation, may have therapeutic value in treating AS patients
Beschreibung:Date Completed 14.03.2023
Date Revised 18.03.2023
published: Print-Electronic
Citation Status MEDLINE
ISSN:1521-7035
DOI:10.1016/j.clim.2023.109268